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Tumor-Promoting Effects of Microrna-421/4-Aminobutyrate Aminotransferase Axis in Hepatocellular Carcinoma
Liu, Yuanguang; Cheng, Ran; Wu, Yijie; Liu, Chunmei; Liu, Yang; Chang, Qing; Yin, Jun.
Afiliação
  • Liu, Yuanguang; Tangshan Gongren Hospital. Department of Hepatobiliary Surgery. Tangshan. CN
  • Cheng, Ran; Tangshan Gongren Hospital. Department of Hepatobiliary Surgery. Tangshan. CN
  • Wu, Yijie; Tangshan Gongren Hospital. Department of Hepatobiliary Surgery. Tangshan. CN
  • Liu, Chunmei; Tangshan Gongren Hospital. Department of Hepatobiliary Surgery. Tangshan. CN
  • Liu, Yang; Tangshan Gongren Hospital. Department of Hepatobiliary Surgery. Tangshan. CN
  • Chang, Qing; Tangshan Gongren Hospital. Department of Head and Neck Surgery. Tangshan. CN
  • Yin, Jun; Tangshan Gongren Hospital. Department of Hepatobiliary Surgery. Tangshan. CN
Rev. invest. clín ; Rev. invest. clín;75(5): 233-248, Sep.-Oct. 2023. tab, graf
Article em En | LILACS-Express | LILACS | ID: biblio-1560108
Biblioteca responsável: MX1.1
ABSTRACT
ABSTRACT

Background:

MicroRNA-421 (miR-421) has been implicated in hepatocellular carcinoma (HCC), but its potential mechanism in HCC remains unclear.

Objectives:

The study aimed to study the potential mechanism of miR-421 in HCC which is necessary.

Methods:

The downstream target genes of miR-421 were screened in HCC tissues and cells using miDIP, Targetscan, and starBase databases. Differential analysis, survival analysis, and Pearson correlation analysis were performed between miR-421 and its downstream target genes. Quantitative reverse transcription polymerase chain reaction and western blot were used to assay RNA and protein levels of 4-aminobutyrate aminotransferase (ABAT) and epithelial-mesenchymal transition (EMT)-related proteins. Cell-based assays, including CCK-8, wound healing, transwell, flow cytometry, and metabolic measurements, were implemented to assess proliferation, migration, invasion, cell cycle, and apoptosis of HCC cells with different treatments. Dual-luciferase assay was utilized to detect the targeting relationship between miR-421 and ABAT.

Results:

miR-421 level was elevated in HCC tissues and cells, and low miR-421 expression hindered phenotype progression of HCC cells. ABAT was identified as a direct target of miR-421 in HCC cells, and miR-421 could inhibit ABAT expression. Rescue assay revealed that miR-421 promoted HCC cell tumorigenesis progress and affected cell metabolic remodeling through down-regulating ABAT.

Conclusion:

The miR-421/ABAT regulatory axis promoted HCC cell tumorigenesis progress, highlighting its potential as a therapeutic target for HCC.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Rev. invest. clín Assunto da revista: MEDICINA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: México
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Rev. invest. clín Assunto da revista: MEDICINA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: México