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Novel approach to soft tissue regeneration: in vitro study of compound hyaluronic acid and horizontal platelet-rich fibrin combination
QIU, Yun; SHEN, Kailun; WEI, Hongjiang; ZHANG, Yufeng; WANG, Yulan; BAI, Yi.
Afiliação
  • QIU, Yun; Wuhan University. School & Hospital of Stomatology. Hubei Key Laboratory of Stomatology. Wuhan. CN
  • SHEN, Kailun; Stomatological Hospital of Xiamen Medical School. Xiamen. CN
  • WEI, Hongjiang; Wuhan University. School & Hospital of Stomatology. Hubei Key Laboratory of Stomatology. Wuhan. CN
  • ZHANG, Yufeng; Wuhan University. School & Hospital of Stomatology. Hubei Key Laboratory of Stomatology. Wuhan. CN
  • WANG, Yulan; Wuhan University. School & Hospital of Stomatology. Hubei Key Laboratory of Stomatology. Wuhan. CN
  • BAI, Yi; Wuhan University. School of Medicine. Medical Research Institute. Wuhan. CN
J. appl. oral sci ; J. appl. oral sci;32: e20230294, 2024. tab, graf
Article em En | LILACS-Express | LILACS | ID: biblio-1558234
Biblioteca responsável: BR1.1
ABSTRACT
Abstract Objective This study aims to develop a compound biomaterial to achieve effective soft tissue regeneration. Methodology Compound hyaluronic acid (CHA) and liquid horizontal-platelet-rich fibrin (H-PRF) were mixed at a ratio of 11 to form a CHA-PRF gel. Human gingival fibroblasts (HGFs) were used in this study. The effect of CHA, H-PRF, and the CHA-PRF gel on cell viability was evaluated by CCK-8 assays. Then, the effect of CHA, H-PRF, and the CHA-PRF gel on collagen formation and deposition was evaluated by qRT‒PCR and immunofluorescence analysis. Finally, qRT‒PCR, immunofluorescence analysis, Transwell assays, and scratch wound-healing assays were performed to determine how CHA, H-PRF, and the CHA-PRF gel affect the migration of HGFs. Results The combination of CHA and H-PRF shortened the coagulation time of liquid H-PRF. Compared to the pure CHA and H-PRF group, the CHA-PRF group exhibited the highest cell proliferation at all time points, as shown by the CCK-8 assay. Col1a and FAK were expressed at the highest levels in the CHA-PRF group, as shown by qRT‒PCR. CHA and PRF could stimulate collagen formation and HGF migration, as observed by fluorescence microscopy analysis of COL1 and F-actin and Transwell and scratch healing assays. Conclusion The CHA-PRF group exhibited greater potential to promote soft tissue regeneration by inducing cell proliferation, collagen synthesis, and migration in HGFs than the pure CHA or H-PRF group. CHA-PRF can serve as a great candidate for use alone or in combination with autografts in periodontal or peri-implant soft tissue regeneration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: J. appl. oral sci Assunto da revista: ODONTOLOGIA Ano de publicação: 2024 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: J. appl. oral sci Assunto da revista: ODONTOLOGIA Ano de publicação: 2024 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil