Your browser doesn't support javascript.
loading
Decreased growth differentiation factor 9, bone morphogenetic protein 15, and forkhead box O3a expressions in the ovary via ulipristal acetate
Fındık, Damla Gül; Kaplanoğlu, Gülnur Take; Arık, Gökçe Nur; Alemari, Nagwa Bugumaa Abubaker.
Afiliação
  • Fındık, Damla Gül; Bilecik Şeyh Edebali University. Faculty of Medicine. Department of Histology and Embryology. Bilecik. TR
  • Kaplanoğlu, Gülnur Take; Gazi University. Faculty of Medicine. Department of Histology and Embryology. Ankara. TR
  • Arık, Gökçe Nur; Gazi University. Faculty of Medicine. Department of Histology and Embryology. Ankara. TR
  • Alemari, Nagwa Bugumaa Abubaker; Gazi University. Faculty of Medicine. Department of Histology and Embryology. Ankara. TR
Rev. Assoc. Med. Bras. (1992, Impr.) ; Rev. Assoc. Med. Bras. (1992, Impr.);69(8): e20230381, 2023. tab, graf
Article em En | LILACS-Express | LILACS | ID: biblio-1507291
Biblioteca responsável: BR1.1
ABSTRACT
SUMMARY

OBJECTIVE:

Folliculogenesis is a complex process involving various ovarian paracrine factors. During folliculogenesis, vitamin D3 and progesterone are significant for the proper development of follicles. This study aimed to investigate the effects of vitamin D3 and selective progesterone receptor modulator ulipristal acetate on ovarian paracrine factors.

METHODS:

In the study, 18 female Wistar-albino rats were randomly divided into three groups control group (saline administration, n=6), vitamin D3 group (300 ng/day vitamin D3 oral administration, n=6), and UPA group (3 mg/kg/day ulipristal acetate oral administration, n=6). Ovarian tissue was analyzed by histochemistry and immunohistochemistry. For quantification of immunohistochemistry, the mean intensities of growth differentiation factor 9, bone morphogenetic protein 15, and forkhead box O3a expressions were measured by Image J and MATLAB. Blood samples were collected for the analysis of serum anti-Müllerian hormone levels by ELISA.

RESULTS:

Atretic follicles and hemorrhagic cystic structures were observed in the UPA group. After immunohistochemistry via folliculogenesis assessment markers, growth differentiation factor 9, bone morphogenetic protein 15, and cytoplasmic forkhead box O3a expressions decreased in the UPA group (p<0.05). Anti-Müllerian hormone level did not differ significantly between the experimental groups (p>0.05).

CONCLUSION:

Ulipristal acetate negatively affects folliculogenesis via ovarian paracrine factors. The recommended dietary vitamin D3 supplementation in healthy cases did not cause a significant change.
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Rev. Assoc. Med. Bras. (1992, Impr.) Assunto da revista: Educa‡Æo em Sa£de / GestÆo do Conhecimento para a Pesquisa em Sa£de / MEDICINA Ano de publicação: 2023 Tipo de documento: Article / Project document País de afiliação: Turquia País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Rev. Assoc. Med. Bras. (1992, Impr.) Assunto da revista: Educa‡Æo em Sa£de / GestÆo do Conhecimento para a Pesquisa em Sa£de / MEDICINA Ano de publicação: 2023 Tipo de documento: Article / Project document País de afiliação: Turquia País de publicação: Brasil