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Overexpression of lncRNA-MEG3 inhibits endometrial cell proliferation and invasion via miR-21-5p/DNMT3B/Twist
Yang, Shaoyan; Feng, Limei; Zhang, Qin; Wu, Lu; Zhao, Qinghua; Hou, Youfang; Yan, Bo; Zhang, Suxian.
Afiliação
  • Yang, Shaoyan; Kunming Medical University. The Second Affiliated Hospital. Department of Gynecology. Wuhua District, Kunming City. CN
  • Feng, Limei; Kunming Medical University. The Second Affiliated Hospital. Department of Pharmacy. Wuhua District, Kunming City. CN
  • Zhang, Qin; Kunming Medical University. The Second Affiliated Hospital. Department of Gynecology. Wuhua District, Kunming City. CN
  • Wu, Lu; Kunming Medical University. The Second Affiliated Hospital. Department of Gynecology. Wuhua District, Kunming City. CN
  • Zhao, Qinghua; Kunming Medical University. The Second Affiliated Hospital. Department of Gynecology. Wuhua District, Kunming City. CN
  • Hou, Youfang; Kunming Medical University. The Second Affiliated Hospital. Department of Gynecology. Wuhua District, Kunming City. CN
  • Yan, Bo; Kunming Medical University. The Second Affiliated Hospital. Department of Gynecology. Wuhua District, Kunming City. CN
  • Zhang, Suxian; Kunming Medical University. The Second Affiliated Hospital. Department of Gynecology. Wuhua District, Kunming City. CN
Clinics ; Clinics;78: 100235, 2023. graf
Article em En | LILACS-Express | LILACS | ID: biblio-1506043
Biblioteca responsável: BR1.1
ABSTRACT
Abstract Recent studies have found that lncRNA-MEG3(MEG3) plays an important role in the development of EMs (Endometriosis), but the specific mechanism needs to be further explored. This study aimed to investigate the effect of MEG3 on the proliferation, invasion of EMs cells. The authors used RT-qPCR to detect the expression of MEG3 and miR-21-5p in EMs tissues and hESCs cells, MTT and Transwell to detect cell proliferation and invasion, western blotting assay to detect the expression of DNMT3B and Twist, MSP to detect the methylation of Twist. The present study's detection results showed that MEG3 was lowly expressed in EMs tissues and hESCs cells, and overexpression of MEG3 could down-regulate miR-21-5p and inhibit endometrial cell proliferation and invasion. In addition, overexpression of MEG3 upregulated the expression of DNMT3B and promoted the methylation of TWIST. In conclusion, the present findings suggest that MEG3 is downregulated in EMs tissues, and overexpression of MEG3 can promote the activity of DNA methyltransferase DNMT3B by downregulating miR-21-5p, thereby promoting the methylation of Twist, downregulating Twist level to inhibits hESCs cells proliferation and invasion.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Clinics Assunto da revista: MEDICINA Ano de publicação: 2023 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Clinics Assunto da revista: MEDICINA Ano de publicação: 2023 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil