Molecular diagnostic of chicken parvovirus (ChPV) affecting broiler flocks in Ecuador
Rev. bras. ciênc. avic
; 20(4): 643-650, Oct.-Dec. 2018. tab, ilus
Article
em En
| VETINDEX
| ID: biblio-1490573
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
Enteric diseases affect poultry and cause important economic losses in many countries worldwide. Avian parvovirus has been linked to enteric conditions, such as malabsorption and runting-stunting syndrome (RSS), characterized by diarrhoea, and reduced weight gain and growth retardation. In 2013 and 2016, 79 samples were collected from different organs of chickens in Ecuador that exhibited signs of diarrhea and stunting syndrome, and analysed for the presence of chicken parvovirus (ChPV). The detection method of ChPV applied was Polymerase Chain Reaction (PCR), using primers designed from the conserved region of the viral genome that encodes the non-structural protein NS1. Out of the 79 samples, 50.6% (40/79) were positive for ChPV, and their nucleotide and amino acid sequences were analysed to determine their phylogenetic relationship with the sequences reported in the United States, Canada, China, South Korea, Croatia, Poland, Hungary, and Brazil. Strong similarity of nucleotide and amino acid sequences among all analyzed sequences and between the analysed and reference sequences was demonstrated, and the phylogenetic analysis clustered all the sequences within the same group, demonstrating a strong relation between the studied strains and the reference chicken parvovirus strains.
Palavras-chave
Texto completo:
1
Base de dados:
VETINDEX
Assunto principal:
Galinhas
/
Parvovirus
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Infecções por Parvoviridae
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Enteropatias
Limite:
Animals
País/Região como assunto:
America do sul
/
Ecuador
Idioma:
En
Revista:
Rev. bras. ciênc. avic
Ano de publicação:
2018
Tipo de documento:
Article
/
Project document