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Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction
Abdel-Rahman, Mohamed A; Abdel-Nabi, Ismail M; El-Naggar, Mohamed S; Abbas, Osama A; Strong, Peter N.
Afiliação
  • Abdel-Rahman, Mohamed A; Suez Canal University. Faculty of Science. Department of Zoology. Ismailia. EG
  • Abdel-Nabi, Ismail M; Port Said University. Faculty of Sciences. Department of Zoology. Port Said. EG
  • El-Naggar, Mohamed S; Suez Canal University. Faculty of Science. Department of Zoology. Ismailia. EG
  • Abbas, Osama A; Port Said University. Faculty of Sciences. Department of Zoology. Port Said. EG
  • Strong, Peter N; Sheffield Hallam University. Biosciences Division. Biomedical Research Center. Sheffield. GB
J. venom. anim. toxins incl. trop. dis ; J. venom. anim. toxins incl. trop. dis;19: 1-10, maio 2013.
Article em En | LILACS, VETINDEX | ID: biblio-1484534
Biblioteca responsável: BR1.1
Localização: BR68.1
ABSTRACT
It is estimated that venoms of marine cone snails (genus Conus) contain more than 100,000 different small peptides with a wide range of pharmacological and biological actions. Some of these peptides were developed into potential therapeutic agents and as molecular tools to understand biological functions of nervous and cardiovascular systems. In this study we examined the cytotoxic and anticancer properties of the marine vermivorous cone snail Conus vexillum (collected from Hurgada and Sharm El-Shaikh, Red Sea, Egypt) and suggest the possible mechanisms involved. The in vitro cytotoxic effects of Conus venom were assessed against Ehrlich’s ascites carcinoma (EAC) cells. Results Conus venom treatment resulted in concentration-dependent cytotoxicity as indicated by a lactate dehydrogenase leakage assay. Apoptotic effects were measured in vivo by measuring levels of reactive oxygen species and oxidative defense agents in albino mice injected with EAC cells. Conus venom (1.25 mg/kg) induced a significant increase ( p  < 0.05) in several oxidative stress biomarkers (lipid peroxidation, protein carbonyl content and reactive nitrogen intermediates) of EAC cells after 3, 6, 9 and 12 hours of venom injection. Conus venom significantly reduced ( p  < 0.05) the activities of oxidative defense enzymes (catalase and superoxide dismutase) as well as the total antioxidant capacity of EAC cells, as evidenced by lowered levels of reduced glutathione.Conclusions These results demonstrate the cytotoxic potential of C. vexillum venom by inducing oxidative stress mediated mechanisms in tumor cells and suggest that the venom contains novel.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Venenos / Carcinoma / Venenos de Moluscos Limite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Egito / Reino Unido País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Venenos / Carcinoma / Venenos de Moluscos Limite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Egito / Reino Unido País de publicação: Brasil