Diluente quimicamente definido e antioxidantes polifenois na criopreservação de sêmen ovino / Chemically defined diluter and polyphenolic antioxidants in the cryopreservation of sheep semen
Ciênc. Anim. (Impr.)
; 30(04, Supl. 2): 294-297, 2020. tab
Article
em Pt
| VETINDEX
| ID: biblio-1472581
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
The objective of this study was to elaborate chemically defined extender based on casein, added with polyphenolic antioxidants, for the freezing of semen from ram breeders. To evaluate Tris-casein (5% glycerol), plus polyphenols, four semen pools from three rams sires were frozen (0µM; 10µM resveratrol; 5µM quercetin; 25µM catechin; 25µM catechin + 10µM resveratrol; 25µM catechin + 5µM quercetin; 10 µM resveratrol + 5µM quercetin; 25µM catechin + 10µM resveratrol + 5µM quercetin). After thawing, the samples were evaluated for sperm kinetics, plasma and acrosomal membrane integrity and mitochondrial membrane potential. There was no significant difference (p≥0.05) between the control group and the treatments for any of the evaluated parameters. However, it can be seen that the Tris-casein extender was efficient in cryopreserving ram semen. It is concluded that the Tris-casein extender can be used for freezing ram semen.
Palavras-chave
Texto completo:
1
Base de dados:
VETINDEX
Assunto principal:
Preservação do Sêmen
/
Ovinos
/
Polifenóis
Limite:
Animals
Idioma:
Pt
Revista:
Ciênc. Anim. (Impr.)
Ano de publicação:
2020
Tipo de documento:
Article
/
Congress and conference