Viability and fertility of stallion semen frozen with ethylene glycol and acetamide as a cryogenic agent
Anim. Reprod. (Online)
; 9(1): 33-39, 2012.
Article
em En
| VETINDEX
| ID: biblio-1461673
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
This study was designed to determine the effect of cooling at a rate of - 0.13 o C/min or any slow cooling prior to freezing on stallion sperm and to determine th e effect of two thawing temperatures on the viability of sperm frozen using a lactose - EDTA - egg yolk extender (LAC) with 3.5% ethylene glycol (LAC 3.5% EG) or a LAC with the additional incorporation of methyl cellulose, trehalose and acetamide (LAC+5% AC). No differences were observed in the post - thaw parameters of sperm frozen using either one of the cryogenic agents or frozen with or without previous slow cooling. Thawing at 75 o C was better than at 37 o C (P < 0.05). An interaction was observed between the c ryoprotectant and the freezing protocol (P < 0.05). A conception rate of 23.3% was obtained after AI using equine semen frozen with LAC+5% AC.
Palavras-chave
Texto completo:
1
Base de dados:
VETINDEX
Assunto principal:
Sêmen
/
Espermatozoides
/
Fertilidade
Limite:
Animals
Idioma:
En
Revista:
Anim. Reprod. (Online)
Ano de publicação:
2012
Tipo de documento:
Article