Impact of an acute heat shock during in vitro maturation on interleukin 6 and its associated receptor component transcripts in bovine cumulus-oocyte complexes
Anim. Reprod. (Online)
; 17(4): e20200221, 2020. tab, graf, ilus
Article
em En
| VETINDEX
| ID: biblio-1461546
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
An acute heat stress event after the LH surge increased interleukin 6 (IL6) levels in the follicular fluid of the ovulatory follicle in hyperthermic cows. To examine direct consequences of a physiologically-relevant elevated temperature (41.0°C) on the cumulus-oocyte complex (COC), IL6 transcript abundance and related receptor components were evaluated throughout in vitro maturation. Heat-induced increases in IL6 were first noted at 4 hours of in vitro maturation (hIVM); peak levels occurred at 4.67 versus 6.44 hIVM for 41.0 and 38.5°C COCs, respectively (SEM = 0.23; P < 0.001). Peak IL6ST levels occurred at 6.95 versus 8.29 hIVM for 41.0 and 38.5°C, respectively (SEM = 0.23; P < 0.01). Transcript for LIF differed over time (P < 0.0001) but was not affected by 41.0°C exposure. Blastocyst development after performing IVF was not affected by 41.0°C exposure for 4 or 6 h. When limiting analysis to when IL6 was temporally produced, progesterone levels were only impacted by time and temperature (no interaction). Heat-induced shift in the temporal production of IL6 and IL6ST along with its impact on progesterone likely cooperate in heat-induced hastening of meiotic progression described by others.
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Texto completo:
1
Base de dados:
VETINDEX
Assunto principal:
Oócitos
/
Resposta ao Choque Térmico
/
Técnicas de Maturação in Vitro de Oócitos
Limite:
Animals
Idioma:
En
Revista:
Anim. Reprod. (Online)
Ano de publicação:
2020
Tipo de documento:
Article