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Usefulness of hemocytometer as a counting chamber in a computer-assisted sperm analyzer (CASA)
Eljarah, A; Chandler, J; Jenkins, J A; Chenevert, J; Alcanal, A.
Afiliação
  • Eljarah, A; Jordan University of Science and Technology. Faculty of Veterinary Medicine. Department of Veterinary Clinical Sciences. Irbid. JO
  • Chandler, J; Louisiana State University. Department of Dairy Science. Baton Rouge. US
  • Jenkins, J A; National Wetlands Research Center. U.S. Geological Survey. Lafayette. BR
  • Chenevert, J; Genez Cooperative Inc. Baton Rouge. US
  • Alcanal, A; Louisiana State University. Department of Dairy Science. Baton Rouge. US
Anim. Reprod. (Online) ; 10(4): 708-711, 2013. tab
Article em En | VETINDEX | ID: biblio-1461092
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Several methods are used to determine sperm cell concentration, such as the haemocytometer, spectrophotometer, electronic cell counter and computer-assisted semen analysers (CASA). The utility of CASA systems has been limited due to the lack of characterization of individual systems and the absence of standardization among laboratories. The aims of this study were to 1) validate and establish setup conditions for the CASA system utilizing the haemocytometer as a counting chamber, and 2) compare the different methods used for the determination of sperm cell concentration in bull semen. Two ejaculates were collected and the soerm cell concentration was detemrined using spectrophotometer and haemocytometer. For the Hamilton-Thorn method, the haemocytometer was used as a counting chamber. Sperm concentration was determined three times per ejaculate sample. A difference (P 0.05) or between the haemocytometer count and the spectrophotometer. Based on the results of this study, we concluded that the haemocytometer can be used in computerized semen analysis systems as a substitute for the commercially available disposable counting chambers, therefore avoiding disadvantageous high costs and slower procedures.
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Sêmen / Hemócitos Limite: Animals Idioma: En Revista: Anim. Reprod. (Online) Ano de publicação: 2013 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Base de dados: VETINDEX Assunto principal: Sêmen / Hemócitos Limite: Animals Idioma: En Revista: Anim. Reprod. (Online) Ano de publicação: 2013 Tipo de documento: Article