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[RETRACTED ARTICLE] lncRNA ZEB1-AS1 inhibits high glucose-induced EMT and fibrogenesis by regulating the miR-216a-5p/BMP7 axis in diabetic nephropathy
Meng, Qingqing; Zhai, Xiaolin; Yuan, Yi; Ji, Qing; Zhang, Pengyuan.
Afiliação
  • Meng, Qingqing; Luoyang Central Hospital Affiliated to Zhengzhou University. Department of Nephrology. Luoyang. CN
  • Zhai, Xiaolin; Luoyang Central Hospital Affiliated to Zhengzhou University. Department of Nephrology. Luoyang. CN
  • Yuan, Yi; Luoyang Central Hospital Affiliated to Zhengzhou University. Department of Nephrology. Luoyang. CN
  • Ji, Qing; Luoyang Central Hospital Affiliated to Zhengzhou University. Department of Nephrology. Luoyang. CN
  • Zhang, Pengyuan; Luoyang Central Hospital Affiliated to Zhengzhou University. Department of Nephrology. Luoyang. CN
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;53(4): e9288, 2020.
Article em En | LILACS-Express | LILACS | ID: biblio-1439698
Biblioteca responsável: BR1.1
ABSTRACT
Diabetic nephropathy (DN) is one of the leading causes of mortality in diabetic patients. Long non-coding RNA zinc finger E-box binding homeobox 1 antisense 1 (ZEB1-AS1) plays a crucial role in the development of various diseases, including DN. However, the molecular mechanism of ZEB1-AS1 in DN pathogenesis remains elusive. An in vitro DN model was established by treating HK-2 cells with high glucose (HG). Quantitative polymerase chain reaction (qRT-PCR) was utilized to detect the expression levels of ZEB1-AS1, microRNA-216a-5p (miR-216a-5p), and bone morphogenetic protein 7 (BMP7). Western blot assay was used to evaluate the protein levels of BMP7, epithelial-to-mesenchymal transition (EMT)-related proteins, and fibrosis markers. Additionally, the interaction among ZEB1-AS1, miR-216a-5p, and BMP7 was predicted by MiRcode (http//www.mircode.org) and starBase 2.0 (omics_06102, omicX), and confirmed by luciferase reporter assay. ZEB1-AS1 and BMP7 were down-regulated, while miR-216a-5p was highly expressed in kidney tissues of DN patients. Consistently, HG treatment decreased the levels of ZEB1-AS1 and BMP7, whereas HG increased miR-216a-5p expression in HK-2 cells in a time-dependent manner. ZEB1-AS1 upregulation inhibited HG-induced EMT and fibrogenesis. Furthermore, ZEB1-AS1 directly targeted miR-216a-5p, and overexpression of miR-216a-5p restored the inhibitory effects of ZEB1-AS1 overexpression on EMT and fibrogenesis. BMP7 was negatively targeted by miR-216a-5p. In addition, ZEB1-AS1 suppressed HG-induced EMT and fibrogenesis by regulating miR-216a-5p and BMP-7. lncRNA ZEB1-AS1 inhibited high glucose-induced EMT and fibrogenesis via regulating miR-216a-5p/BMP7 axis in diabetic nephropathy, providing a potential target for DN therapy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Tipo de estudo: Prognostic_studies Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Tipo de estudo: Prognostic_studies Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China País de publicação: Brasil