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In vitro establishment and multiplication of Echynochloa polystachya
Silveira, Emanuel Orestes da; Lopes, Ricardo; Rodrigues, Filipe Almendagna; Raizer, Marcelo Domingues Martins; Dias, Fabio Jacobs; Harada, Pamela Keiko; Araújo, Daniele Simões; Pasqual, Moacir; Luz, José Magno Queiroz; Lopes, Maria Teresa Gomes.
Afiliação
  • Silveira, Emanuel Orestes da; Federal University of Amazonas. Parintins. BR
  • Lopes, Ricardo; Embrapa Western Amazon. Manaus. BR
  • Rodrigues, Filipe Almendagna; Federal University of Lavras. Lavras. BR
  • Raizer, Marcelo Domingues Martins; Embrapa Western Amazon. Manaus. BR
  • Dias, Fabio Jacobs; Federal University of Amazonas. Manaus. BR
  • Harada, Pamela Keiko; Embrapa Western Amazon. Manaus. BR
  • Araújo, Daniele Simões; Embrapa Western Amazon. Manaus. BR
  • Pasqual, Moacir; Federal University of Lavras. Lavras. BR
  • Luz, José Magno Queiroz; Federal University of Uberlândia. Uberlândia. BR
  • Lopes, Maria Teresa Gomes; Federal University of Amazonas. Uberlândia. BR
Biosci. j. (Online) ; 38: e38075, Jan.-Dec. 2022. ilus, graf
Article em En | LILACS | ID: biblio-1397078
Biblioteca responsável: BR396.1
ABSTRACT
The objective of this work was to carry out the in vitro establishment of Echynochloa polystachya aiming at obtaining a micropropagation protocol for works involving the selection of superior genotypes and the cultivation of the species. E. polystachya stems were collected in the municipality of Manaus-AM. Explants were inoculated in test tubes containing Murashige and Skoog (MS) medium. Thirty days after in vitro establishment, the rate of sprouting and contamination were evaluated. Experiments were also carried out to assess the effects of sucrose and 6-benzylaminopurine (BAP) concentrations on the tillering rate of explants. It was found that during the successive subcultures there was a decrease in internodes and the consequent loss of vigor. There were responses in the multiplication rate at concentrations starting from 45 g L-1 sucrose. In addition, BAP and sucrose interfered the development and in vitro multiplication. Sucrose in conjunction with BAP was harmful and shortened internodes. The physiological state of the explants for the species under study was intrinsically linked to the concentrations of sucrose used for the culture medium and the concentrations of BAP. However, the sucrose and BAP concentrations suggested for in vitro cultivation of E. Polystachya must be adjusted during successive subcultures. Absence of contamination in the in vitro establishment occurred at concentrations 15, 30 and 60 g L-1 sucrose. The combination of 1.5 mg L-1 BAP and 30 g L-1 sucrose promoted greater induction of sprouts. In addition, the in vitro rooting of E. polystachya was 45%.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Técnicas In Vitro / Brachiaria / Técnicas de Cultura de Tecidos Idioma: En Revista: Biosci. j. (Online) Assunto da revista: Agricultura / Disciplinas das Ciˆncias Biol¢gicas / Pesquisa Interdisciplinar Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Técnicas In Vitro / Brachiaria / Técnicas de Cultura de Tecidos Idioma: En Revista: Biosci. j. (Online) Assunto da revista: Agricultura / Disciplinas das Ciˆncias Biol¢gicas / Pesquisa Interdisciplinar Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil