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Evaluation of the in vitrofungicidal activity of the dynamized essential oil of Aloysia polystachyabefore and after freezing
Nader, Talita Thomaz; Henrique, Camila Yamasita; Leonel, Alexandre Henrique; Pereira, Ana Maria Soares.
Afiliação
  • Nader, Talita Thomaz; Homeopatia Brasil. s.l. BR
  • Henrique, Camila Yamasita; Homeopatia Brasil. s.l. BR
  • Leonel, Alexandre Henrique; Homeopatia Brasil. s.l. BR
  • Pereira, Ana Maria Soares; Universidade de Ribeirão Preto. Ribeirão Preto. BR
Int. j. high dilution res ; 21(1): 12-12, May 6, 2022.
Article em En | LILACS, HomeoIndex | ID: biblio-1396583
Biblioteca responsável: BR926.1
ABSTRACT
The method of preserving substances of natural origin should not only maintain the microbiological safety of the product but also the integrity of its therapeutic potential. Essential oils obtained from plants are complex mixtures of substances and it issuggested to keep them under refrigeration for better conservation. On the other hand, homeopathic mother tincture prepared from plant is kept at room temperature.

Aim:

This work aimed to evaluate if the freezing process changes the in vitro antifungal activity potential of the homeopathic preparation Aloysia polystachya1CH against Candida albicans.

Methodology:

The inoculum of C. albicansATCC 10231 was cultivated in culture médium Sabouroud (Himedia®), standardized on a spectrometer and distributed in a 96-well plate. Then, A. polystachya1CH was added to the wells, prepared accordingtothe Brazilian Homeopathic Pharmacopoeia (FHB, 3rd edition) from A. polystachya essencial oil. An aliquot of this homeopathic preparation was frozen and after 40 days it was submitted to the same methodology for evaluation of the antifungal activity. After incubation, the plates were read with triphenyltetrazolic (TTC) (Vetec®). Results and

discussion:

The results of the in vitroevaluation showed that the freezing process retained the antifungal activity of the dynamized essential oil of A. polystachya1CH against C. albicans.

Conclusion:

Under the conditions evaluated in this study, the freezing method presented as a viable method of conservation of dynamized plant material.
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Texto completo: 1 Coleções: 01-internacional Base de dados: HomeoIndex / LILACS Assunto principal: Técnicas In Vitro / Candida albicans / Preparações Derivadas / Antifúngicos Idioma: En Revista: Int. j. high dilution res Assunto da revista: HOMEOPATIA / Terapias Complementares Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: HomeoIndex / LILACS Assunto principal: Técnicas In Vitro / Candida albicans / Preparações Derivadas / Antifúngicos Idioma: En Revista: Int. j. high dilution res Assunto da revista: HOMEOPATIA / Terapias Complementares Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil