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Molecular signature of immunological mechanism behind impaired endometrial receptivity in polycystic ovarian syndrome
Amjadi, Fatemehsadat; Zandieh, Zahra; Mehdizadeh, Mehdi; Ajdary, Marziyeh; Aghamajidi, Azin; Raoufi, Ehsan; Aflatoonian, Reza.
Afiliação
  • Amjadi, Fatemehsadat; Iran University of Medical Science. Akbarabadi Hospital. Akbarabadi IVF clinic. Tehran. IR
  • Zandieh, Zahra; Iran University of Medical Science. Akbarabadi Hospital. Akbarabadi IVF clinic. Tehran. IR
  • Mehdizadeh, Mehdi; Iran University of Medical Sciences. Department of Anatomical Sciences. Tehran. IR
  • Ajdary, Marziyeh; Iran University of Medical Sciences. Endometriosis Research Center. Tehran. IR
  • Aghamajidi, Azin; Iran University of Medical Sciences. School of Medicine. Department of Immunology. Tehran. IR
  • Raoufi, Ehsan; Iran University of Medical Sciences. School of Allied Medicine. Department of Medical Biotechnology. Tehran. IR
  • Aflatoonian, Reza; Royan Institute for Reproductive Biomedicine. Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center. Tehran. IR
Arch. endocrinol. metab. (Online) ; 66(3): 303-311, June 2022. tab, graf
Article em En | LILACS-Express | LILACS | ID: biblio-1393861
Biblioteca responsável: BR1.1
ABSTRACT
ABSTRACT

Objective:

Despite the treatment of anovulation, infertility is still one of the main complications in PCOS women during reproductive age, which appears to be mainly due to impaired uterine receptivity. This study investigated the transcriptome profiles of endometrium in PCOS patients and healthy fertile individuals as the control group. Material and

methods:

Total mRNA was extracted from endometrial tissues of PCOS patients (n = 12) and healthy fertile individuals (n = 10) during the luteal phase. After cDNA synthesis, PCR array was performed using Human Female Infertility RT² Profiler PCR Array kit (Qiagen, Cat. No PAHS-164Z) for evaluating expression of 84 genes contributing to the female infertility.

Results:

PCR Array data analysis identified significantly greater expression of CSF, IL11, IL15, IL1r1, IL1b, TNF, LIF, TNFRSF10B, TGFβ, C3, ITGA4 (Cd49d), SPP1, and Calca in PCOS women than in controls (P < 0.05). However, the expression of LIFR, C2, CD55, CFD, CALCA, LAM1, LAMC2, MMP2, MMP7, MMP9, ESR, SELL, ITGB3, and VCAM1 was significantly lower in PCOS group than in controls (P < 0.05). The results revealed dysregulation of immune-inflammatory molecules, complement activation and downregulation of IGF-I as well as adhesion molecules in PCOS group.

Conclusion:

The findings of this study indicated some potential causes of reduced receptivity of endometrium thus compromising the fertility in PCOS patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Tipo de estudo: Prognostic_studies Idioma: En Revista: Arch. endocrinol. metab. (Online) Assunto da revista: ENDOCRINOLOGIA / METABOLISMO Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Tipo de estudo: Prognostic_studies Idioma: En Revista: Arch. endocrinol. metab. (Online) Assunto da revista: ENDOCRINOLOGIA / METABOLISMO Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil