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Efficacy of Ficus carica leaf extract on morphological and molecular behavior of mice germ stem cells
Makoolati, Zohreh; Bahrami, Hasan; Zamanzadeh, Zahra; Mahaldashtian, Maryam; Moulazadeh, Alireza; Ebrahimi, Lida; Naghdi, Majid.
Afiliação
  • Makoolati, Zohreh; Fasa University of Medical Sciences. Faculty of Medicine. Department of Anatomical Sciences. Fasa. IR
  • Bahrami, Hasan; Nour Danesh Institute of higher Education. Basic Sciences Faculty. Department of Genetic. Isfahan. IR
  • Zamanzadeh, Zahra; Shahid Ashrafi Esfahani University. Faculty of Biological Sciences and Technology. Department of Biotechnology. Isfahan. IR
  • Mahaldashtian, Maryam; Fasa University of Medical Sciences. Faculty of Medicine. Department of Anatomical Sciences. Fasa. IR
  • Moulazadeh, Alireza; Fasa University of Medical Sciences. Non Communicable Disease Research Center. Fasa. IR
  • Ebrahimi, Lida; Fasa University of Medical Sciences. School of Advanced Technologies. Department of Tissue Engineering. Fasa. IR
  • Naghdi, Majid; Fasa University of Medical Sciences. Faculty of Medicine. Department of Anatomical Sciences. Fasa. IR
Anim. Reprod. (Online) ; 19(2): e20220036, mai. 2022. graf, tab, ilus
Article em En | VETINDEX | ID: biblio-1393185
Biblioteca responsável: BR68.1
ABSTRACT
Infertility is one of the most prevalent health disorders in reproductive-age males and females. Ficus carica (Fc), an herbal plant, has been used traditionally for the treatment of different diseases such as infertility especially in Iranian folk medicine. This study examined the effects of Fc leaf extract on the proliferation of mice spermatogonial stem cells (SSCs). Phenolic, flavonoid content, major polyphenolic compounds and antioxidant activity of the extract was evaluated respectively by Folin-Ciocateu, aluminum chloride, HPLC and the FRAP and DPPH methods. Testicular cells of neonate mice were extracted and their identity was confirmed using cytokeratin for Sertoli and Oct-4, CDHI and PLZF for SSCs. Effects of Fc (0.0875, 0.175, 0.35, 0.71 and 1.42 mg/ml) was evaluated at third, 7th, 9th and 14th days of culture by colony assay. The expression of the Mvh, GFRα1 and Oct-4 genes and the viability and proliferation of cultured cells was assessed at the end of the culture period. The extract has a rich phenolic and flavonoid content such as Rutin, Psoralen, Bergapten and Caffeoylmalic acid using HPLC analysis. It also had a potent reducing and radical scavenging activity. Morphology of colonies was similar in all groups. Higher viability, proliferation, colony number and diameter of SSCs was seen in the presence of Fc leaf extract in a dosedependent manner so that higher number and diameter of colonies were observed in two higher doses of 0.71 and 1.42 mg/ml, separately for each time point relative to other groups. The Mvh, Oct-4 and GFRα1 genes expression had no significant differences between groups. It seems that Fc leaf extract not only had no any cytotoxic effects on the viability and proliferation of SSCs but also support their stemness state. So, this culture system can be employed for enrichment of germ stem cells for use in clinical applications.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Extratos Vegetais / Ficus / Camundongos Limite: Animals Idioma: En Revista: Anim. Reprod. (Online) Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Extratos Vegetais / Ficus / Camundongos Limite: Animals Idioma: En Revista: Anim. Reprod. (Online) Ano de publicação: 2022 Tipo de documento: Article