Compositional and toxicological investigation of pooled venom from farm-raised Naja atra
J. venom. anim. toxins incl. trop. dis
; J. venom. anim. toxins incl. trop. dis;28: e20210040, 2022. tab, graf, ilus
Article
em En
| LILACS, VETINDEX
| ID: biblio-1365074
Biblioteca responsável:
BR68.1
ABSTRACT
Background:
Naja atra is a venomous snake species medically relevant in China. In the current study, we evaluated the composition and toxicological profile of venom collected from farm-raised N. atra.Methods:
Venom was collected from third-generation captive bred N. atra on a snake farm in Hunan Province, China. The venom was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis and nano-liquid chromatography with electrospray ionization tandem mass spectrometry. In addition, hemolytic activity, median lethal dose, serum biochemical and histopathological parameters were accessed.Results:
N. atra venom proteome was dominated by phospholipase A2 (46.5%) and three-finger toxins (41.4 %), and a set of common low relative abundance proteins, including cysteine-rich secretory proteins (4.7%), NGF-beta (2.4%), snake venom metalloproteinase (1.5%), glutathione peroxidase (0.6%), vespryn (0.3%), and 5ʹ-nucleotidases (0.2%) were also found. Furthermore, the venom exhibited direct hemolytic activity, neurotoxicity, myotoxicity, and high lethal potency in mice, with a subcutaneous median lethal dose of 1.02 mg/kg. Histopathological analysis and serum biochemical tests revealed that venom caused acute hepatic, pulmonary and renal injury in mice.Conclusion:
This study revealed the composition and toxicity of venom collected from farm-raised N. atra, thereby providing a reference for the analysis of venom samples collected from captive-born venomous snakes in the future.(AU)Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
/
VETINDEX
Assunto principal:
Venenos de Serpentes
/
Fosfolipases A2
/
Naja naja
/
Miotoxicidade
Limite:
Animals
Idioma:
En
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
Brasil