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Compositional and toxicological investigation of pooled venom from farm-raised Naja atra
Xiao, Gang; Liu, Junqi; Peng, Lingfeng; Yang, Yang; Sun, Zhiliang.
Afiliação
  • Xiao, Gang; Hunan Agricultural University. College of Veterinary Medicine. Changsha. CN
  • Liu, Junqi; Hunan Agricultural University. College of Veterinary Medicine. Changsha. CN
  • Peng, Lingfeng; Hunan Agricultural University. College of Veterinary Medicine. Changsha. CN
  • Yang, Yang; Hunan Agricultural University. College of Veterinary Medicine. Changsha. CN
  • Sun, Zhiliang; Hunan Agricultural University. College of Veterinary Medicine. Changsha. CN
J. venom. anim. toxins incl. trop. dis ; J. venom. anim. toxins incl. trop. dis;28: e20210040, 2022. tab, graf, ilus
Article em En | LILACS, VETINDEX | ID: biblio-1365074
Biblioteca responsável: BR68.1
ABSTRACT

Background:

Naja atra is a venomous snake species medically relevant in China. In the current study, we evaluated the composition and toxicological profile of venom collected from farm-raised N. atra.

Methods:

Venom was collected from third-generation captive bred N. atra on a snake farm in Hunan Province, China. The venom was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis and nano-liquid chromatography with electrospray ionization tandem mass spectrometry. In addition, hemolytic activity, median lethal dose, serum biochemical and histopathological parameters were accessed.

Results:

N. atra venom proteome was dominated by phospholipase A2 (46.5%) and three-finger toxins (41.4 %), and a set of common low relative abundance proteins, including cysteine-rich secretory proteins (4.7%), NGF-beta (2.4%), snake venom metalloproteinase (1.5%), glutathione peroxidase (0.6%), vespryn (0.3%), and 5ʹ-nucleotidases (0.2%) were also found. Furthermore, the venom exhibited direct hemolytic activity, neurotoxicity, myotoxicity, and high lethal potency in mice, with a subcutaneous median lethal dose of 1.02 mg/kg. Histopathological analysis and serum biochemical tests revealed that venom caused acute hepatic, pulmonary and renal injury in mice.

Conclusion:

This study revealed the composition and toxicity of venom collected from farm-raised N. atra, thereby providing a reference for the analysis of venom samples collected from captive-born venomous snakes in the future.(AU)
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Venenos de Serpentes / Fosfolipases A2 / Naja naja / Miotoxicidade Limite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Venenos de Serpentes / Fosfolipases A2 / Naja naja / Miotoxicidade Limite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Brasil