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A biocomplex to repair experimental critical size defects associated with photobiomodulation therapy
Buchaim, Daniela Vieira; Andreo, Jesus Carlos; Pomini, Karina Torres; Barraviera, Benedito; Ferreira Júnior, Rui Seabra; Duarte, Marco Antonio Hungaro; Alcalde, Murilo Priori; Reis, Carlos Henrique Bertoni; Teixeira, Daniel de Bortoli; Bueno, Cleuber Rodrigo de Souza; Detregiachi, Cláudia Rucco Penteado; Araujo, Adriano Cressoni; Buchaim, Rogério Leone.
Afiliação
  • Buchaim, Daniela Vieira; University of Marilia. Marília. BR
  • Andreo, Jesus Carlos; University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences. Bauru. BR
  • Pomini, Karina Torres; University of Marilia. Marília. BR
  • Barraviera, Benedito; São Paulo State University. Center for the Study of Venoms and Venomous Animals. Botucatu. BR
  • Ferreira Júnior, Rui Seabra; São Paulo State University. Center for the Study of Venoms and Venomous Animals. Botucatu. BR
  • Duarte, Marco Antonio Hungaro; University of São Paulo. Bauru School of Dentistry. Department of Dentistry, Endodontics and Dental Materials. Bauru. BR
  • Alcalde, Murilo Priori; Unisagrado University Center. Department of Health Science. Bauru. BR
  • Reis, Carlos Henrique Bertoni; University of Marilia. Marília. BR
  • Teixeira, Daniel de Bortoli; University of Marilia. Marília. BR
  • Bueno, Cleuber Rodrigo de Souza; University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences. Bauru. BR
  • Detregiachi, Cláudia Rucco Penteado; University of Marilia. Marília. BR
  • Araujo, Adriano Cressoni; University of Marilia. Marília. BR
  • Buchaim, Rogério Leone; São Paulo State University. Center for the Study of Venoms and Venomous Animals. Botucatu. BR
J. venom. anim. toxins incl. trop. dis ; J. venom. anim. toxins incl. trop. dis;28: e20210056, 2022. tab, graf, ilus
Article em En | LILACS, VETINDEX | ID: biblio-1360567
Biblioteca responsável: BR68.1
ABSTRACT
The association of scaffolds to repair extensive bone defects can contribute to their evolution and morphophysiological recomposition. The incorporation of particulate biomaterials into three-dimensional fibrin bioproducts together with photobiomodulation therapy (PBM) has potential and can improve regenerative medicine procedures. The objective of this experiment was to evaluate the effects of PBM therapy on critical size defects filled with xenogenic bone substitute associated with fibrin biopolymer.

Methods:

A critical defect of 8 mm was performed in 36 Wistar male adult rats that were divided into four groups. Groups BC and BC-PBM were defined as controls with defects filled by a clot (without or with PBM, respectively) and groups XS and XS-PBM that comprised those filled with biocomplex Bio-OssTM in association with fibrin biopolymer. PBM was applied immediately after the surgery and three times a week every other day, with the parameters wavelength of 830 nm, energy density 6.2 J/cm2, output power 30 mW, beam area of 0.116 cm2, irradiance 0.258,62 W/cm2, energy/point 0.72 J, total energy 2.88 J. Fourteen and 42 days after the surgery, animals were euthanatized and subjected to microtomography, qualitative and quantitative histological analysis.

Results:

The BC-PBM and XS-PBM groups had a similar evolution in the tissue repair process, with a higher density of the volume of new formed bone in relation to the groups without PBM (p = 0.04086; p = 0.07093, respectively). Intense vascular proliferation and bone deposition around the biomaterial particles were observed in the animals of the groups in which biocomplex was applied (XS and XS-PBM).

Conclusion:

PBM therapy allowed an improvement in the formation of new bone, with a more organized deposition of collagen fibers in the defect area. Biocomplex favored the insertion and permanence of the particulate material in bone defects, creating a favorable microenvironment for accelerate repair process.(AU)
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Materiais Biocompatíveis / Biopolímeros / Colágeno / Terapia com Luz de Baixa Intensidade Tipo de estudo: Qualitative_research / Risk_factors_studies Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Materiais Biocompatíveis / Biopolímeros / Colágeno / Terapia com Luz de Baixa Intensidade Tipo de estudo: Qualitative_research / Risk_factors_studies Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil