Brain-derived Neurotropic factor (BDNF) mediates the protective effect of Cucurbita pepo L. on salivary glands of rats exposed to chronic stress evident by structural, biochemical and molecular study

Almohaimeed, Hailah M; Albadawi, Emad A; Mohammedsaleh, Zuhair M; Alghabban, Hadel M; Seleem, Hanan S; Ramadan, Osama I; Ayuob, Nasra N

Almohaimeed, Hailah M; Albadawi, Emad A; Mohammedsaleh, Zuhair M; Alghabban, Hadel M; Seleem, Hanan S; Ramadan, Osama I; Ayuob, Nasra N.

Afiliação

Almohaimeed, Hailah M; Princess Nourah bint Abdulrahman University. College of Medicine. Department of Basic Science. Riyadh. SA
Albadawi, Emad A; Taibah University. College of Medicine. Department of Anatomy. SA
Mohammedsaleh, Zuhair M; University of Tabuk. Faculty of Applied Medical Sciences. Department of Medical Laboratory Technology. Tabuk. SA
Alghabban, Hadel M; University of Taibah. Faculty of Applied Medical Sciences. Department of Medical Laboratory Technology. SA
Seleem, Hanan S; Menoufia University. Faculty of Medicine. Department of Histology. Shebin ElKoum. EG
Ramadan, Osama I; Al Azhar University. Damietta Faculty of Medicine. Histology Department. Cairo. EG
Ayuob, Nasra N; Damietta University. Faculty of Medicine. Department of Medical Histology. Damietta. EG

J. appl. oral sci ; J. appl. oral sci;29: e20201080, 2021. tab, graf

Article em En | LILACS | ID: biblio-1340115

Biblioteca responsável: BR1.1

ABSTRACT

ABSTRACT

Abstract Acute and chronic stresses affect the salivary glands, representing the source of plasma BDNF during stressful conditions. Pumpkin is a medicinal plant with an evident antioxidant, anti-inflammatory and potential antidepressant effects. Objective To assess the structural and biochemical effects induced by exposure to chronic unpredictable mild stress (CUMS) on salivary glands of albino rats, and to evaluate the role of pumpkin extract (Pump) in ameliorating this effect. Methodology Four groups (n=10 each) of male albino rats were included in this study the control, CUMS, Fluoxetine-treated and Pump-treated. The corticosterone, the pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), and the oxidant/antioxidant profile were all assessed in the serum. The level of BDNF mRNA was measured in the salivary glands using qRT-PCR. Histopathological changes of the salivary glands were also assessed. Results The depressive-like status was confirmed behaviorally and biochemically. Exposure to CUMS significantly up-regulated (p<0.001) the level of serum corticosterone. CUMS induced degenerative changes in the secretory and ductal elements of the salivary glands evident by increased apoptosis. Both Fluoxetine and Pumpkin significantly up-regulated (p<0.001) BDNF expression in the salivary glands and ameliorated the CUMS-induced histopathological and biochemical alterations in the salivary glands. Pumpkin significantly (p<0.001) increased the serum levels of antioxidant enzymes SOD, GPX and CAT, and reduced the serum levels of the pro-inflammatory cytokines TNF-α, IL-6. Conclusion Pumpkin ameliorates the depressive-like status induced in rats following exposure to chronic stress through exerting a promising anti-inflammatory, antioxidant and anti-depressant-like effects. The pumpkin, subsequently, improved stress-induced structural changes in the salivary glands that might be due to up-regulation of BDNF expression in the glands.

Assuntos

Animais; Ratos; Encéfalo; Glândulas Salivares; Cucurbita

Palavras-chave

BDNF; Chronic stress; Depression; Fluoxetine; Parotid gland; Pumpkin; Submandibular gland

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Encéfalo Limite: Animals Idioma: En Revista: J. appl. oral sci Assunto da revista: ODONTOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Egito / Arábia Saudita País de publicação: Brasil

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