Development of a species-specific PCR assay for authentication of Agkistrodon acutus based on mitochondrial cytochrome b gene
Electron. j. biotechnol
; 49: 29-33, Jan. 2021. tab, ilus
Article
em En
| LILACS
| ID: biblio-1291632
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Agkistrodon acutus, a traditional Chinese medicine, clinically used in the treatment of rheumatism, tumor, and cardiovascular and cerebrovascular diseases. Due to the unique medicinal value and the difficulty of artificial breeding of Agkistrodon acutus, the supply of Agkistrodon acutus on the market exceeds the demand, and a large number of its adulterants are found on the market. In this study, the cytb gene sequences of Agkistrodon acutus and 9 snakes were compared and analyzed, specific primers were designed, and specific PCR methods were established to detect Agkistrodon acutus medicinal samples on the market.RESULTS:
This method was successfully applied to distinguish the snake from other adulterated species, and tested 18 Agkistrodon acutus samples randomly purchased from six cities. Twelve samples were counterfeit and six were genuine. The standard reference material of Agkistrodon acutus was cloned by molecular cloning and sequencing, and the gene sequence difference with other species was significant. It shows that the region could be used as the fingerprint region of the target species.CONCLUSIONS:
The proposed method can be used as a species-specific marker and can be highly distinguished from other adulterated snake species, which is helpful to effectively avoid the problem of false sale of Agkistrodon acutus.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Reação em Cadeia da Polimerase
/
Agkistrodon
/
Citocromos b
/
Mitocôndrias
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Electron. j. biotechnol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China