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Sucrose, Lactose, and Xylitol Exposures Affect Biofilm Formation of Streptococcus mutans
Nuraini, Prawati; Kriswandini, Indah Listiana; Ridwan, Rini Devijanti; Soetjipto,.
Afiliação
  • Nuraini, Prawati; Airlangga University. Pediatric Dentistry Department. Faculty of Dental Medicine. Surabaya. ID
  • Kriswandini, Indah Listiana; Airlangga University. Oral Biology Department. Faculty of Dental Medicine. Surabaya. ID
  • Ridwan, Rini Devijanti; Airlangga University. Oral Biology Department. Faculty of Dental Medicine. Surabaya. ID
  • Soetjipto,; Airlangga University. Medical Biochemistry Department. Faculty of Medicine. Surabaya. ID
Article em En | LILACS, BBO | ID: biblio-1287491
Biblioteca responsável: BR1264.1
ABSTRACT
Abstract

Objective:

To determine the level of biofilm formation of S. mutans after being exposed to 5% sucrose, 8% lactose, or 1% xylitol. Material and

Methods:

This research was a laboratory-based experimental study with post-test only control group design. S. mutans was grown in test tubes containing tryptose soy broth (TSB) medium supplemented with 1% glucose. They were incubated at 37° C for 24 hours to grow the biofilms. The culture was then exposed to 5% sucrose, 8% lactose or 1% xylitol, incubated for 24 hours at 37° C, and examined using ELISA at a wavelength of 625 nm. The statistical analysis was performed using a one-way analysis of variance followed by the least significant difference test (a=0.05).

Results:

There were some differences in the biofilm formation of S. mutans after exposure to 5% sucrose, 8% lactose, or 1% xylitol (p<0.05). An LSD test indicated significant differences among the biofilm formations after exposure to 5% sucrose and 8% lactose and between 5% sucrose and 1% xylitol. In comparison, there were no significant differences (p>0.05) between 8% lactose and 1% xylitol.

Conclusion:

Sucrose, lactose and xylitol can form biofilms and the formation of lactose biofilms is the same as xylitol.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: BBO / LILACS Assunto principal: Streptococcus mutans / Sacarose / Xilitol / Dissacarídeos / Indonésia País/Região como assunto: Asia Idioma: En Revista: Pesqui. bras. odontopediatria clín. integr Assunto da revista: ODONTOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Indonésia País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: BBO / LILACS Assunto principal: Streptococcus mutans / Sacarose / Xilitol / Dissacarídeos / Indonésia País/Região como assunto: Asia Idioma: En Revista: Pesqui. bras. odontopediatria clín. integr Assunto da revista: ODONTOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Indonésia País de publicação: Brasil