Tissue specificity in rat peripheral nerve regeneration through combined skelatal muscle and vein conduit grafts
s.l; s.n; 2000. 7 p. ilus, tab.
Non-conventional
em En
| SES-SP, SESSP-ILSLACERVO, SES-SP
| ID: biblio-1242731
Biblioteca responsável:
BR191.1
Localização: [{"text": "BR191.1", "_a": "09224/s"}]
ABSTRACT
Diffusible factors from the distal stumps of transected peripheral nerves exert a neurotropic effect on regenerating nerves in vivo (specificity). This morphologial study was designed to investigate the existence of tissue specificity in peripheral nerve fiber regeneration through a graft of vein filled with fresh skeletal muscle. This tubulization technique demonstrated experiemtal and clinical results similar to those obtained with traditional autologous nerve grafts. Specifically, we used Y-shaped grafts to assess the orientation pattern of regenerating axons in the distal tissue. Animal models were divided into four experimental groups. The proximal part of the Y-shaped conduit was sutured to a severed tibial nerve in all experiments. The two distal stumps were sutured ot different targets group A to two intact nerves (tibial and peroneal), group B to tan intact nerve and an unvascularized tendon, group C to an intact nerve and a vascularized tendon, and group D to a nerve graft and an unvascularized tendon. Morphological evaluation by light and electron microscopy was conducted in the distal forks of the Y-shaped tube. Data showed that almost all regenerating nerve fibers spontaneously oriented towards the nerve tissue (attached or not the peripheral innervation field), showing a good morphological pattern of regeneration in both the early and late phases of regeneration. When the distal choice was represented by a tendon (vascularized or not), very few nerve fibers were detected in the corresponding distal fork of the Y-shaped graft. These results show that, using the musclevein-combined grafting technique, regenerating axons are able to correctly grow and orientate within the basement membranes of the graft guided by the neurotropic lure of the distal nerve stump
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Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-ILSLACERVO
Assunto principal:
Osso e Ossos
/
Sistema Nervoso Periférico
Limite:
Humans
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Non-conventional