Profiles of the mRNA expression by macrophages infected with mycobacterium leprae and mycobacterium avium complex
Int. j. lepr. other mycobact. dis
; 70(4): 250-259, Dec., 2002. ilus, tab
Article
em En
| SES-SP, HANSEN, HANSENIASE, SESSP-ILSLACERVO, SES-SP
| ID: biblio-1227121
Biblioteca responsável:
BR191.1
Localização: [{"text": "BR191.1"}]
ABSTRACT
In the present study, we examined profiles of the interaction of Mycobacterium leprae and Mycobacterium avium complex (MAC) with murine peritoneal macrophages (M phi s) in terms of up-regulation of M phi expression of proinflammatory and immunosuppressing cytolines (CKs) after infection. First, the reverse transcription polymerase chain reaction (RT-PCR) assay revealed that both MAC and M. leprae infections up-regulated M phi mRNA expression IL-12, TNF-alpha, IL-10, and transformating growth factor-beta (TGF-beta), except that M. leprae-infected M phi s showed no increase in the IL-12 mRNA expression. Second, the RT-PCR assay also showed some differences between M. leprae- and MAC-infected M phi s with respect to the modes of IL-10 and inducible nitric oxide synthase (iNOS) mRNA expression. That is MAC, but not M. leprae, infection caused a prolonged increase in the expression of IL-10 and iNOS mRNAs. Third, a ribonuclease protection assay revealed that M phi s co-infected with MAC and M. leprae showed the Il-12, TNF-alpha and IL-10 mRNA expression in an intermediate mode of those of M phi s infected with either M. leprae or MAC alone. This implies that the CK expression of M. leprae-infected M phi s may be modified by co-infection with MAC. These findings may suggest differential interactions of M. leprae and MAC organisms with murine peritoneal M phi s in terms of the activation of signal transduction pathways for expression of some kinds of immunoregulatory cytokines and immunoprotective enzymes.
Texto completo:
1
Coleções:
06-national
/
BR
Base de dados:
HANSEN
/
HANSENIASE
/
SES-SP
/
SESSP-ILSLACERVO
Assunto principal:
RNA
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Macrófagos
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Mycobacterium avium
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Mycobacterium leprae
Idioma:
En
Revista:
Int. j. lepr. other mycobact. dis
Ano de publicação:
2002
Tipo de documento:
Article