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Profiles of the mRNA expression by macrophages infected with mycobacterium leprae and mycobacterium avium complex
Shimizu, Toshiaki; Tomioka, Haruaki; Matsuoka, Masanori; Sano, Chiaki.
Afiliação
  • Shimizu, Toshiaki; Shimane Medical University. Department of Microbiology and Immunology. JP
  • Tomioka, Haruaki; Shimane Medical University. Department of Microbiology and Immunology. JP
  • Matsuoka, Masanori; National Institute of Infectious Diseases. Leprosy Research Center. Tokyo. JP
  • Sano, Chiaki; Shimane Medical University. Department of Microbiology and Immunology. JP
Int. j. lepr. other mycobact. dis ; 70(4): 250-259, Dec., 2002. ilus, tab
Article em En | SES-SP, HANSEN, HANSENIASE, SESSP-ILSLACERVO, SES-SP | ID: biblio-1227121
Biblioteca responsável: BR191.1
Localização: [{"text": "BR191.1"}]
ABSTRACT
In the present study, we examined profiles of the interaction of Mycobacterium leprae and Mycobacterium avium complex (MAC) with murine peritoneal macrophages (M phi s) in terms of up-regulation of M phi expression of proinflammatory and immunosuppressing cytolines (CKs) after infection. First, the reverse transcription polymerase chain reaction (RT-PCR) assay revealed that both MAC and M. leprae infections up-regulated M phi mRNA expression IL-12, TNF-alpha, IL-10, and transformating growth factor-beta (TGF-beta), except that M. leprae-infected M phi s showed no increase in the IL-12 mRNA expression. Second, the RT-PCR assay also showed some differences between M. leprae- and MAC-infected M phi s with respect to the modes of IL-10 and inducible nitric oxide synthase (iNOS) mRNA expression. That is MAC, but not M. leprae, infection caused a prolonged increase in the expression of IL-10 and iNOS mRNAs. Third, a ribonuclease protection assay revealed that M phi s co-infected with MAC and M. leprae showed the Il-12, TNF-alpha and IL-10 mRNA expression in an intermediate mode of those of M phi s infected with either M. leprae or MAC alone. This implies that the CK expression of M. leprae-infected M phi s may be modified by co-infection with MAC. These findings may suggest differential interactions of M. leprae and MAC organisms with murine peritoneal M phi s in terms of the activation of signal transduction pathways for expression of some kinds of immunoregulatory cytokines and immunoprotective enzymes.
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Texto completo: 1 Coleções: 06-national / BR Base de dados: HANSEN / HANSENIASE / SES-SP / SESSP-ILSLACERVO Assunto principal: RNA / Macrófagos / Mycobacterium avium / Mycobacterium leprae Idioma: En Revista: Int. j. lepr. other mycobact. dis Ano de publicação: 2002 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 06-national / BR Base de dados: HANSEN / HANSENIASE / SES-SP / SESSP-ILSLACERVO Assunto principal: RNA / Macrófagos / Mycobacterium avium / Mycobacterium leprae Idioma: En Revista: Int. j. lepr. other mycobact. dis Ano de publicação: 2002 Tipo de documento: Article