Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G> A with Orofacial Cleft
Pesqui. bras. odontopediatria clín. integr
; 21: e5896, 2021. tab, graf
Article
em En
| LILACS, BBO
| ID: biblio-1180869
Biblioteca responsável:
BR1264.1
ABSTRACT
ABSTRACT Objective:
To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material andMethods:
A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme. The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test.Results:
The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group.Conclusion:
The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
BBO
/
LILACS
Assunto principal:
Polimorfismo Genético
/
Variação Genética
/
Fenda Labial
/
Fissura Palatina
/
Metilenotetra-Hidrofolato Desidrogenase (NADP)
Tipo de estudo:
Evaluation_studies
/
Risk_factors_studies
País/Região como assunto:
Asia
Idioma:
En
Revista:
Pesqui. bras. odontopediatria clín. integr
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Indonésia
País de publicação:
Brasil