Purificacion y estudio enzimatico de los quininogenos I y II del plasma humano / [Purification and enzyme study of kininogens I and II from human plasma]
Acta physiol. latinoam
; 26(4): 248-53, 1976.
Article
em Es
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LILACS-Express
| BINACIS
| ID: biblio-1158478
Biblioteca responsável:
AR1.1
Localização: [{"text": "AR1.1"}]
RESUMO
Kininogens I (HMW) and II (LMW) were isolated and partially purified from human plasma. The disappearance of kininogen I was prevented by the use of hexadimethrine bromide, which inhibits the activation of plasma prekallikrein. The two kininogens were separated by chromatography on DEAE-cellulose. Further purification of kininogen I and II was achieved by separate chromatographic steps of the partially purified kininogens on SP-Sephadex. The purification of the kininogens was controlled by incubation of the respective samples with different kininogenases human plasma kallikrein, human salivary kallikrein and trypsin. As determined by gel filtration, a molecular weight of 250 000 daltons was found for kininogen I (HMW) and 48 000 daltons for kininogen II (LMW).
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Coleções:
06-national
/
AR
Base de dados:
BINACIS
Idioma:
Es
Revista:
Acta physiol. latinoam
Ano de publicação:
1976
Tipo de documento:
Article
País de publicação:
Argentina