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Phenotypic and genotypic analysis of biofilm production by Pseudomonas aeruginosa isolates from infection and colonization samples
Rodrigues, Rodrigo Lira; Lima, Jailton Lobo da Costa; Sena, Kêsia Xisto da Fonseca Ribeiro de; Maciel, Maria Amélia Vieira.
Afiliação
  • Rodrigues, Rodrigo Lira; Universidade Federal de Pernambuco. Centro de Ciências Médicas. Coordenação de Área Medicina Tropical. Recife. BR
  • Lima, Jailton Lobo da Costa; Universidade Federal de Pernambuco. Centro de Ciências Médicas. Coordenação de Área Medicina Tropical. Recife. BR
  • Sena, Kêsia Xisto da Fonseca Ribeiro de; Universidade Federal de Pernambuco. Centro de Biociências. Departamento de Antibióticos. Recife. BR
  • Maciel, Maria Amélia Vieira; Universidade Federal de Pernambuco. Centro de Ciências Médicas. Coordenação de Área Medicina Tropical. Recife. BR
Rev. Soc. Bras. Med. Trop ; Rev. Soc. Bras. Med. Trop;53: e20200399, 2020. tab, graf
Article em En | SES-SP, ColecionaSUS, LILACS | ID: biblio-1136908
Biblioteca responsável: BR1.1
ABSTRACT
Abstract

INTRODUCTION:

Pseudomonas aeruginosa is an opportunistic pathogen associated with healthcare-related infections, affecting mainly patients with underlying diseases and immunosuppression. This microorganism has several virulence mechanisms that favour its pathogenesis, including the production of biofilm. This study aimed to analyze the phenotypic production of biofilms, the occurrence of quorum sensing (QS) genes, and the clonal profile of clinical isolates of P. aeruginosa from colonized/infected patients in a tertiary hospital in Recife-PE.

METHODS:

We obtained 21 isolates that were classified as infection isolates (II), and 10 colonization isolates (CI). The phenotypic analysis for biofilm production was performed quantitatively. The QS genes were detected by specific PCRs, and the clonal profile was assessed using ERIC-PCR.

RESULTS:

Of the 31 isolates, 58.1 % (18/31) were biofilm producers, of which 70 % (7/10) were CI and classified as weakly adherent; 52.4 % (11/21) of the II produced biofilms, and were classified as weak (38.1 %, (8/21)), moderate (9.5 %, (2/21)), and strongly adherent (4.8 %, (1/21)). All isolates harbored the QS genes analyzed. In the clonal analysis, 26 distinct genetic profiles were identified, highlighting the presence of a clone in four samples, i.e., one infection isolate, and 3 colonization isolates.

CONCLUSIONS:

The detection of biofilm formation is important in P. aeruginosa in addition to the identification of colonization and infection isolates, especially from complex environments such as ICUs. Further, we define a strategy for monitoring and analyzing P. aeruginosa strains that can potentially cause infections in hospitalized patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / SES-SP / ColecionaSUS Assunto principal: Pseudomonas aeruginosa / Infecções por Pseudomonas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Rev. Soc. Bras. Med. Trop Assunto da revista: MEDICINA TROPICAL Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / SES-SP / ColecionaSUS Assunto principal: Pseudomonas aeruginosa / Infecções por Pseudomonas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Rev. Soc. Bras. Med. Trop Assunto da revista: MEDICINA TROPICAL Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil