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Qualitative and quantitative molecular analysis of bacteria in root canals of primary teeth with pulp necrosis
Lemos, Samira Salomão; Cesar, Dionéia Evangelista; ProcÓpio, Stefânia Werneck; Machado, Fernanda Campos; Ribeiro, Luiz Claudio; Ribeiro, Rosangela Almeida.
Afiliação
  • Lemos, Samira Salomão; Universidade Federal de Juiz de Fora. Postgraduate Program in Dentistry. Juiz de Fora. BR
  • Cesar, Dionéia Evangelista; Universidade Federal de Juiz de Fora. Biological Sciences Institute. Department of Ecology and Molecular Biology of Microorganisms. Juiz de Fora. BR
  • ProcÓpio, Stefânia Werneck; Universidade Federal de Minas Gerais. School of Dentistry. Postgraduate Program in Dentistry. Belo Horizonte. BR
  • Machado, Fernanda Campos; Universidade Federal de Juiz de Fora. School of Dentistry. Department of Social and Child Dentistry. Juiz de Fora. BR
  • Ribeiro, Luiz Claudio; Universidade Federal de Juiz de Fora. Department of Statistics. Juiz de Fora. BR
  • Ribeiro, Rosangela Almeida; Universidade Federal de Juiz de Fora. School of Dentistry. Department of Social and Child Dentistry. Juiz de Fora. BR
Braz. oral res. (Online) ; 34: e093, 2020. tab, graf
Article em En | LILACS, BBO | ID: biblio-1132652
Biblioteca responsável: BR1.1
ABSTRACT
Abstract Information about bacterial diversity, such as the number of each species in the root canals of primary teeth, contributes to improving our effective management of infections of endodontic origin in primary teeth. This study made a qualitative and quantitative assessment of the bacteria in the root canals of primary teeth with necrotic pulp, using the fluorescence in situ hybridization (FISH) technique. Thirty-one primary teeth with pulp necrosis from 31 children were evaluated using the FISH technique, to detect the presence and density of Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Enterococcus faecalis, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Streptococcus, Streptococcus mutans, Streptococcus sobrinus, Tannerella forsythia and Treponema denticola. Descriptive measures explained the data related to density, and Student's t-test assessed the differences among the densities of each bacterium, according to signs and symptoms. The bacterial density was paired and correlated. All bacteria tested were detected and identified in all the samples. The average number of bacterial individuals from each species ranged from 1.9 x 108 cells/mL (S. mutans) to 3.1 x 108 cells/mL (F. nucleatum) (p > 0.05). The sum of the mean counts of each bacterium represented almost 80% of the entire microbial community. Patients with pain had significantly more T. denticola, and those with edema showed a greater density of Streptococcus and P. nigrescens (p < 0.05). This study revealed that all 12 bacteria evaluated were found in all primary teeth with pulp necrosis. There was no predominance among the species studied; all species had a similar number of individuals.
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Texto completo: 1 Coleções: 01-internacional Base de dados: BBO / LILACS Assunto principal: Dente Decíduo / Necrose da Polpa Dentária / Cavidade Pulpar Tipo de estudo: Qualitative_research Limite: Child / Humans Idioma: En Revista: Braz. oral res. (Online) Assunto da revista: ODONTOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: BBO / LILACS Assunto principal: Dente Decíduo / Necrose da Polpa Dentária / Cavidade Pulpar Tipo de estudo: Qualitative_research Limite: Child / Humans Idioma: En Revista: Braz. oral res. (Online) Assunto da revista: ODONTOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil