Loop-mediated isothermal amplification (LAMP) assay for rapid and accurate confirmatory diagnosis of HTLV-1/2 infection
Viruses
; 12(981): 1-15, 2020. ilus
Article
em En
| LILACS, SES-SP, SESSP-IALPROD, SES-SP
| ID: biblio-1122302
Biblioteca responsável:
BR91.2
Localização: BR76.1; P
ABSTRACT
Laboratory diagnosis of human T-lymphotropic viruses (HTLV) 1 and 2 infection is performed by serological screening and further confirmation with serological or molecular assays. Thus, we developed a loop-mediated isothermal nucleic acid amplification (LAMP) assay for the detection of HTLV-1/2 in blood samples. The sensitivity and accuracy of HTLV-1/2 LAMP were defined with DNA samples from individuals infected with HTLV-1 (n = 125), HTLV-2 (n = 19), and coinfected with HIV (n = 82), and compared with real-time polymerase chain reaction (qPCR) and PCR-restriction fragment length polymorphism (RFLP). The overall accuracy of HTLV-1/2 LAMP (95% CI 74.885.5%) was slightly superior to qPCR (95% CI 69.581.1%) and similar to PCR-RFLP (95% CI 79.589.3%). The sensitivity of LAMP was greater for HTLV-1 (95% CI 83.293.4%) than for HTLV-2 (95% CI 43.270.8%). This was also observed in qPCR and PCR-RFLP, which was associated with the commonly lower HTLV-2 proviral load. All molecular assays tested showed better results with samples from HTLV-1/2 mono-infected individuals compared with HIV-coinfected patients, who present lower CD4 T-cell counts. In conclusion, HTLV-1/2 LAMP had similar to superior performance than PCR-based assays, and therefore may represent an attractive alternative for HTLV-1/2 diagnosis due to reduced working time and costs, and the simple infrastructure needed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
/
SES-SP
/
SESSP-IALPROD
Assunto principal:
Vírus
/
DNA
/
Vírus Linfotrópico T Tipo 1 Humano
/
Vírus Linfotrópico T Tipo 2 Humano
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Viruses
Assunto da revista:
Biologia
/
Virologia
/
Viroses
/
V¡rus
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Suíça