Assessment of pectinase-producing fungi isolated from soil and the use of orange waste as a substrate for pectinase production
Rev. ciênc. farm. básica apl
; Rev. ciênc. farm. básica apl;4001/01/2019. ilus, tab
Article
em En
| LILACS
| ID: biblio-1100196
Biblioteca responsável:
BR33.1
ABSTRACT
Pectinases are important enzymes not only for their potential applications in different industries such animal feed, agricultural, textile, beverage, food processing, oil extraction, etc. Ten fungal species were isolated from the soil and screened for production of pectinase enzyme by using the pectin agar medium. Pectinolytic enzymes synthesis were attained at a temperature of 30 °C and activities were determined after a seven-days culture of Aspergillus sp. 391 and Aspergillus sp. 031, in a basic medium containing 2% citrus pectin and as the sole carbon source. The extract enzymatic showed an optimum activity for exo-polygalacturonase (PG) and pectin lyase (PNL) against galacturonic acid and pectin at pH 4.5 and 5.5, respectively. There were variations in PG and PNL enzymes levels produced in culture filtrates obtained of Aspergillus sp. 391 with addition of citrus waste (2.0 and 4.0 % w/v) to the medium. Maximum activity for PNL activity was observed in the medium containing 5% pectin or 4% citrus waste, as sole carbon source, after 7 days of growth. The results showed that the isolate Aspergillus sp. 391 is a promising for pectinolytic enzymes production at the industrial level.(AU)
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Poligalacturonase
/
Aspergillus
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Citrus sinensis
Idioma:
En
Revista:
Rev. ciênc. farm. básica apl
Assunto da revista:
FARMACOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil