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Synergistic effect of microRNA and albumin-bound nanoparticles for inhibition of glioblastoma cancer cell proliferation
Shariatnasery, Maria; Irani, Shiva; Soleimani, Masoud; Goodarzi, Navid; Dinarvand, Rassoul.
Afiliação
  • Shariatnasery, Maria; Islamic Azad University. Science and Research Branch. Department of Biology. Tehran. IR
  • Irani, Shiva; Islamic Azad University. Science and Research Branch. Department of Biology. Tehran. IR
  • Soleimani, Masoud; Tarbiat Modares University. School of Medical Sciences. Tehran. IR
  • Goodarzi, Navid; Tehran University of Medical Sciences. Faculty of Pharmacy. Nanotechnology Research Centre. Tehran. IR
  • Dinarvand, Rassoul; Tehran University of Medical Sciences. Faculty of Pharmacy. Nanotechnology Research Centre. Tehran. IR
Braz. J. Pharm. Sci. (Online) ; 56: e18306, 2020. tab, graf
Article em En | LILACS-Express | LILACS | ID: biblio-1089224
Biblioteca responsável: BR1.1
ABSTRACT
The functional significance of upregulation miR-34a in combination with albumin-bound paclitaxel nanoparticles in U251 glioblastoma cell line has been evaluated. The MTT assay determined that miR-34a and albumin-bound paclitaxel nanoparticles can reduce cell viability, but the combination of both factors has a stronger effect on cell viability. The application of qRT-PCR has demonstrated that the transduction of miR-34a could lead to exogenous upregulation of miR-34a level and downregulation of SURVIVIN. Moreover, treatment of U251 cells with miR-34a and nanoparticles together considerably inhibit SURVIVIN expression compared to miR-34a and nanoparticles alone. Flow cytometry showed that upon miR-34a overexpression cell cycle arrested in G1 phase, while treatment with nanoparticles increased the cell population in G2 phase. Upregulation of miR-34a along with treatment with nanoparticles elevated the number of cells arrested in G1/ G2 phases of the cell cycle. Expression of miR-34a with albumin-bound paclitaxel nanoparticles reduced cell viability, downregulated SURVIVIN and enhanced cell cycle arrest in G1/G2 phases. Thus, the upregulation of miR-34a with these nanoparticles are potential candidates therapeutic for glioblastoma cancer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Braz. J. Pharm. Sci. (Online) Assunto da revista: Farmacologia / Terapˆutica / Toxicologia Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Braz. J. Pharm. Sci. (Online) Assunto da revista: Farmacologia / Terapˆutica / Toxicologia Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil