Your browser doesn't support javascript.
loading
A nested polymerase chain reaction test for bovine coronavirus diagnosis bases on the RNA-dependent RNA-polymerase gene
Brandão, PE; Gregori, F; Villarreal, LYB; Rosales, CAR; Soares, RM; Jerez, JA.
Afiliação
  • Brandão, PE; Secretaria de Estado da Saúde de São Paulo. Instituto Pasteur. São Paulo. BR
  • Gregori, F; Instituto Biológico. São Paulo. BR
  • Villarreal, LYB; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
  • Rosales, CAR; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
  • Soares, RM; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
  • Jerez, JA; Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. São Paulo. BR
Virus review and research ; 10(1): 45-49, sept. 2005. tab
Article em En | SES-SP, SESSP-IPPROD, SES-SP | ID: biblio-1068425
Biblioteca responsável: BR84.1
Localização: BR 84.1
ABSTRACT
Bovine coronavirus (BCoV) causes enteritis and respiratory iIIness in calves and dysentery in cows. Difficulties found in the production of large amounts of BCoV in cel! culture and those regarding envelope integrity pose a major problem to antigen, hyperimmune sera and monoclonal antibodies production. This study aimed at the development of a polymerase chain reaction for BCoV detection in fecal samples of calves targeted to the gene of the RNA-dependent RNApolymerase, and the comparison of this test with the hemagglutination/ hemagglutination inhibition test (HA/HI). Both tests were applied to 203 fecal samples. HA/HI resulted in an individual-levei BCoV frequency of 35.47% and a farm-Ievel frequency of 73.68%, while the proposed PCR resulted in an individual-levei BCoV frequency of 25.12% and a farm-Ievel frequency of 52.63%. Both Kappa and Youden's tests revealed a low agreement between PCR and HA/HI. Hypotheses are suggested to explain such a disagreement. The PCR proposed is a useful toei forthe detection of BCoV, for epidemiological surveys and as a screening test for studies focused on other BCoV genes, such as the spike gene.
Assuntos
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IPPROD Assunto principal: Reação em Cadeia da Polimerase / Coronavirus Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Virus review and research Ano de publicação: 2005 Tipo de documento: Article
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IPPROD Assunto principal: Reação em Cadeia da Polimerase / Coronavirus Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Virus review and research Ano de publicação: 2005 Tipo de documento: Article