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Modified toxin-binding inhibition (ToBI) test for epsilon antitoxin determination in serum of immunized rabbits
Sobrinho, Eliane M; Cangussu, Alex S R; Brandi, Igor V; Sari, Roger S; Almeida, Anna C; Colen, Fernando; Quintilio, Wagner; Santos, Hercules O.
Afiliação
  • Sobrinho, Eliane M; s.af
  • Cangussu, Alex S R; s.af
  • Brandi, Igor V; s.af
  • Sari, Roger S; s.af
  • Almeida, Anna C; s.af
  • Colen, Fernando; s.af
  • Quintilio, Wagner; Instituto Butantan. São Paulo. BR
  • Santos, Hercules O; s.af
Veterinary Immunology and Immunopathology ; 138(1-2): 129-133, 2010.
Article em En | SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO | ID: biblio-1068396
Biblioteca responsável: BR78.1
Localização: BR78.1
ABSTRACT
The aim of the present study was to evaluate and standardize the ToBI test in vitro as a substitute for the serum neutralization test in mice for quality control of clostridial vaccines. The ToBI test in vitro was used to evaluate 40 serum samples of known antibody content, obtained from rabbits immunized against clostridiosis with experimental vaccine. The correlation between epsilon antitoxin titers in rabbit sera, determined by the ToBI test and serum neutralization in mice, ranged from 0.222% to 0.452% in polyvalent vaccines and from 0.154% to 0.387% in monovalent vaccines. Interplate coefficients of variation were not significant, reaching 0.350% in polyvalent vaccines and 0.400% in monovalent vaccines, indicating high homogeneity. In conclusion, the ToBI test in vitro is suitable for assessing the potency of clostridial vaccines and may be used as an alternative method able to replace current in vivo tests.
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Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Antitoxinas / Clostridium perfringens Limite: Animals Idioma: En Revista: Veterinary Immunology and Immunopathology Ano de publicação: 2010 Tipo de documento: Article
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Antitoxinas / Clostridium perfringens Limite: Animals Idioma: En Revista: Veterinary Immunology and Immunopathology Ano de publicação: 2010 Tipo de documento: Article