Analysis of polymorphisms in Plasmodium falciparum genes related to drug resistance: a survey over four decades under different treatment policies in Brazil
Malaria Journal
; 13(372): 1-10, Set, 2014. map, tab, graf
Article
em En
| SES-SP, SESSP-SUCENPROD, SES-SP
| ID: biblio-1064641
Biblioteca responsável:
BR93.2
Localização: BR93.2
ABSTRACT
Anti-malarial resistance in Plasmodium falciparum remains an obstacle for malaria control. Resistance-associated genes were analysed in Brazilian samples over four decades to evaluate the impact of different treatment regimens on the parasite genetic profile. Methods:
Samples were collected on filter paper from patients infected in the Amazon region from 1984 to 2011.DNA was extracted with Chelex® 100 and monoinfection confirmed by PCR. SNPs in the pfcrt, pfmdr1, pfdhfr and pfdhpsgenes were assessed by PCR-RFLP. The pfmdr1 copy number was estimated using real time quantitative PCR with SYBR®Green. Parasite response was assessed ex vivo with seven concentrations of each anti-malarial. Patients were treatedaccording to Brazilian guidelines quinine plus tetracycline or mefloquine in period 1 and ACT in period 2...Palavras-chave
Texto completo:
1
Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-SUCENPROD
Assunto principal:
Plasmodium falciparum
Tipo de estudo:
Guideline
Limite:
Humans
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Malaria Journal
Ano de publicação:
2014
Tipo de documento:
Article