Ferromagnetic resonance for the quantification of superparamagnetic iron oxide nanoparticles in biological materials
Int. j. nanomed. (Online)
; 5: 203-211, 2010.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1063573
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
The aim of the present work is the presentation of a quantification methodology for the control of the amount of superparamagnetic iron oxide nanoparticles (SPIONs) administeredin biological materials by means of the ferromagnetic resonance technique (FMR) applied to studies both in vivo and in vitro. The in vivo study consisted in the analysis of the eliminationand biodistribution kinetics of SPIONs after intravenous administration in Wistar rats. The results were corroborated by X-ray fluorescence. For the in vitro study, a quantitative analysisof the concentration of SPIONs bound to the specific AC133 monoclonal antibodies was carriedout in order to detect the expression of the antigenic epitopes (CD133) in stem cells from human umbilical cord blood. In both studies FMR has proven to be an efficient technique forthe SPIONs quantification per volume unit (in vivo) or per labeled cell (in vitro).
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Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Assunto principal:
Espectroscopia de Ressonância Magnética
/
Nanopartículas
Limite:
Animals
Idioma:
En
Revista:
Int. j. nanomed. (Online)
Ano de publicação:
2010
Tipo de documento:
Article