Differentially expressed genes in the liver of thioacetamide treated rats
Comp. biochem. physiol., C Comp. pharmacol
; 24(18): 129-135, jun.2003.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1062148
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
Thioacetamide is a hepatotoxic and hepatocarcinogenic compound that affects liver metabolism, inhibits mRNA transport and induces enlargement of the nucleolus. To investigate the effect of thioacetamide at the molecular level, differential display RT-PCR was conducted. Analysis of nineteen differentially expressed genes demonstrated that ten cDNAs have their expression inhibited while the other nine were positively affected by thioacetamide. Two of the cDNAs were homologous to known genes-TAP and ankyrin-binding glycoprotein-1, two corresponded to repetitive sequences and seven were homologous to expressed sequence tags. The differential expression of some of the isolated cDNAs was confirmed by northern hybridization. It is proposed that since the product of TAP is involved in mRNA transport, thioacetamide inhibition of TAP expression might, at least partially, explain the thioacetamide-induced swelling of the nucleolus.
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Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Assunto principal:
Tioacetamida
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RNA Mensageiro
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Fígado
Limite:
Animals
Idioma:
En
Revista:
Comp. biochem. physiol., C Comp. pharmacol
Ano de publicação:
2003
Tipo de documento:
Article