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Lipopolysaccharide as an Antigen Target for the Formulation of a Universal Vaccine against Escherichia coli O111 Strains
Santos, Maurílio F; New, Roger R. C; Andrade, Gabrielle R; Ozaki, Christiane Y; Sant’Anna, Osvaldo A; Mendonça Previato, Lucia; Trabulsi, Luis R; Domingos, Marta O.
Afiliação
  • Santos, Maurílio F; Instituto Butantan. São Paulo. BR
  • New, Roger R. C; s.af
  • Andrade, Gabrielle R; Instituto Butantan. São Paulo. BR
  • Ozaki, Christiane Y; Instituto Butantan. São Paulo. BR
  • Sant’Anna, Osvaldo A; Instituto Butantan. São Paulo. BR
  • Mendonça Previato, Lucia; s.af
  • Trabulsi, Luis R; Instituto Butantan. São Paulo. BR
  • Domingos, Marta O; Instituto Butantan. São Paulo. BR
Clín. méd. H.C.C ; 17(11): 1772-1780, Nov.2010.
Article em En | SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO | ID: biblio-1062120
Biblioteca responsável: BR78.1
Localização: BR78.1
ABSTRACT
A promising approach to developing a vaccine against O111 strains of diarrheagenic Escherichia coli thatexhibit different mechanisms of virulence is to target either the core or the polysaccharide chain (O antigen)of their lipopolysaccharide (LPS). However, due to structural variations found in both these LPS components,to use them as antigen targets for vaccination, it is necessary to formulate a vaccine able to induce a humoralimmune response that can recognize all different variants found in E. coli O111 strains. In this study, it was demonstrated that, despite differences in composition of oligosaccharide repeat units between O111ab and O111ac LPS subtypes, antibodies against one O111 subtype can recognize and inhibit the adhesion to human epithelial cells of all categories of O111 E. coli(enteropathogenic E. coli [EPEC], enterohemorrhagic E. coli [EHEC], and enteroaggregative E. coli [EAEC]) strains regardless of the nature of their flagellar antigens, mechanisms of virulence, or O111 polysaccharide subtypes. These antibodies were also able to increase the clearance of different strains of O111 E. coli by macrophages. PCR analyses of the pathways involved in O111 LPS core biosynthesis showed that all EAEC strains have core type R2, whereas typical EPEC and EHEC havecore type R3. In contrast, atypical EPEC strains have core types R2 and R3. In summary, the results presentedherein indicate that the O111 polysaccharide and LPS core types R2 and R3 are antigen targets for panspecific immunotherapy against all categories of O111 E. coli.
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Texto completo: 1 Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Lipopolissacarídeos / Escherichia coli Limite: Animals Idioma: En Revista: Clín. méd. H.C.C Ano de publicação: 2010 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
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Texto completo: 1 Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Lipopolissacarídeos / Escherichia coli Limite: Animals Idioma: En Revista: Clín. méd. H.C.C Ano de publicação: 2010 Tipo de documento: Article