Enhancement of the citrullinenitric oxide cycle inastroglioma cells by the proline-rich peptide-10c from Bothrops jararaca venom
Brain res
; Exp. brain res;1363(6): 11-19, Sept. 25,2010.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1061595
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
The biological activity of the proline-rich decapeptide Bj-PRO-10c, a processing product of the C-type natriuretic peptide precursor protein, expressed in the brain and the venom gland of the pit viper Bothrops jararaca, was originally attributed to the inhibition of the somatic angiotensin-converting enzyme activity with subsequent anti-hypertensive effect. However, recent results suggest broader biological activity may also be involved in the cardiovascular effects of this peptide. Here we show that Bj-PRO-10c enhances and sustains the generation of nitric oxide (NO) by regulating argininosuccinate synthase activity and thereby velocity of the citrullineNO cycle. Bj-PRO-10c-mediated effects not restricted to thecardiovascular system, since NO production was also induced in cells of astroglial origin. Bj-PRO-10c was internalized by C6 astroglioma cells where it induces NO production and upregulation of the citrullineNO cycle cells in a dose-dependent fashion. In view of that, astroglial cells function as L-arginine pool for NO production in neighboring neurons, we suggest a regulatory function for Bj-PRO-10c on the metabolism of this gaseous neurotransmitter in the CNS. Moreover, proliferation of astroglial cells was reduced in the presence of Bj-PRO-10c; however, cell death was not induced. Since NO donors have been studied for the treatment of solid cancers, Bj-PRO-10c may serve as structural model fordeveloping drugs to improve the effects of cancer therapy based on the peptide's ability to augment NO production.
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Coleções:
06-national
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BR
Base de dados:
SES-SP
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SESSP-IBACERVO
/
SESSP-IBPROD
Assunto principal:
Prolina
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Bothrops
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Anti-Hipertensivos
Limite:
Animals
Idioma:
En
Revista:
Brain res
/
Exp. brain res
Ano de publicação:
2010
Tipo de documento:
Article