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Enhancement of the citrulline–nitric oxide cycle inastroglioma cells by the proline-rich peptide-10c from Bothrops jararaca venom
Oliveira, Eduardo Fontana de; Guerreiro, Juliano R; Silva, Carlos A; Benedetti, Gabriel Fernando de Souza; Lebrun, Ivo; Ulrich, Henning; Lameu, Claudiana; Camargo, Antonio C . M.
Afiliação
  • Oliveira, Eduardo Fontana de; Instituto Butantan. São Paulo. BR
  • Guerreiro, Juliano R; s.af
  • Silva, Carlos A; s.af
  • Benedetti, Gabriel Fernando de Souza; Instituto Butantan. São Paulo. BR
  • Lebrun, Ivo; Instituto Butantan. São Paulo. BR
  • Ulrich, Henning; s.af
  • Lameu, Claudiana; Instituto Butanan. São Paulo. BR
  • Camargo, Antonio C . M; Instituto Butantan. São Paulo. BR
Brain res ; Exp. brain res;1363(6): 11-19, Sept. 25,2010.
Article em En | SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO | ID: biblio-1061595
Biblioteca responsável: BR78.1
Localização: BR78.1
ABSTRACT
The biological activity of the proline-rich decapeptide Bj-PRO-10c, a processing product of the C-type natriuretic peptide precursor protein, expressed in the brain and the venom gland of the pit viper Bothrops jararaca, was originally attributed to the inhibition of the somatic angiotensin-converting enzyme activity with subsequent anti-hypertensive effect. However, recent results suggest broader biological activity may also be involved in the cardiovascular effects of this peptide. Here we show that Bj-PRO-10c enhances and sustains the generation of nitric oxide (NO) by regulating argininosuccinate synthase activity and thereby velocity of the citrulline–NO cycle. Bj-PRO-10c-mediated effects not restricted to thecardiovascular system, since NO production was also induced in cells of astroglial origin. Bj-PRO-10c was internalized by C6 astroglioma cells where it induces NO production and upregulation of the citrulline–NO cycle cells in a dose-dependent fashion. In view of that, astroglial cells function as L-arginine pool for NO production in neighboring neurons, we suggest a regulatory function for Bj-PRO-10c on the metabolism of this gaseous neurotransmitter in the CNS. Moreover, proliferation of astroglial cells was reduced in the presence of Bj-PRO-10c; however, cell death was not induced. Since NO donors have been studied for the treatment of solid cancers, Bj-PRO-10c may serve as structural model fordeveloping drugs to improve the effects of cancer therapy based on the peptide's ability to augment NO production.
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Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Prolina / Bothrops / Anti-Hipertensivos Limite: Animals Idioma: En Revista: Brain res / Exp. brain res Ano de publicação: 2010 Tipo de documento: Article
Buscar no Google
Adicionar na Minha BVS
Imprimir
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Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Prolina / Bothrops / Anti-Hipertensivos Limite: Animals Idioma: En Revista: Brain res / Exp. brain res Ano de publicação: 2010 Tipo de documento: Article