Effect of medium composition on the production of tetanus toxin by Clostridium tetani
Biotechnology Progress
; 21(3): 756-761, 2005.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1060894
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
The tetanus toxin is a neurotoxin synthesized by the bacillus Clostridium tetani that, after detoxification with formaldehyde, still exhibits antigenic and immunologic properties, hence its denomination of tetanus toxoid. Such a neurotoxin is produced by cultivation of the microorganism in vegetative form on a relatively complex specific medium containing glucose and peptone. The simultaneous effects of the starting levels of glucose (G0) and N-Z Case TT (NZ0) as carbon and nitrogen sources, respectively, on the production of tetanus toxin have been investigated in this work in static cultivations by means of a five-level star-shaped experimental design and evaluated by response surface methodology (RSM) for optimization purposes. The highest final average yield of tetanus toxin (72 Lf/mL), achieved at G0= 9.7 g/L and NZ0= 43.5 g/L, was 80% higher than that obtained with standard cultivations (G0= 8.0 g/L and NZ0= 25.0 g/L).
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Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Assunto principal:
Tétano
/
Toxoide Tetânico
Limite:
Humans
Idioma:
En
Revista:
Biotechnology Progress
Ano de publicação:
2005
Tipo de documento:
Article