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Effect of medium composition on the production of tetanus toxin by Clostridium tetani
Fratelli, F; Siquini, T J; Prado, S M A; Higashi, H G; Converti, A; de Carvalho, J C M.
Afiliação
  • Fratelli, F; Instituto Butantan. São Paulo. BR
  • Siquini, T J; Instituto Butantan. São Paulo. BR
  • Prado, S M A; Instituto Butantan. São Paulo. BR
  • Higashi, H G; Instituto Butantan. São Paulo. BR
  • Converti, A; s.af
  • de Carvalho, J C M; s.af
Biotechnology Progress ; 21(3): 756-761, 2005.
Article em En | SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO | ID: biblio-1060894
Biblioteca responsável: BR78.1
Localização: BR78.1
ABSTRACT
The tetanus toxin is a neurotoxin synthesized by the bacillus Clostridium tetani that, after detoxification with formaldehyde, still exhibits antigenic and immunologic properties, hence its denomination of tetanus toxoid. Such a neurotoxin is produced by cultivation of the microorganism in vegetative form on a relatively complex specific medium containing glucose and peptone. The simultaneous effects of the starting levels of glucose (G0) and N-Z Case TT (NZ0) as carbon and nitrogen sources, respectively, on the production of tetanus toxin have been investigated in this work in static cultivations by means of a five-level star-shaped experimental design and evaluated by response surface methodology (RSM) for optimization purposes. The highest final average yield of tetanus toxin (72 Lf/mL), achieved at G0= 9.7 g/L and NZ0= 43.5 g/L, was 80% higher than that obtained with standard cultivations (G0= 8.0 g/L and NZ0= 25.0 g/L).
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Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Tétano / Toxoide Tetânico Limite: Humans Idioma: En Revista: Biotechnology Progress Ano de publicação: 2005 Tipo de documento: Article
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
Coleções: 06-national / BR Base de dados: SES-SP / SESSP-IBACERVO / SESSP-IBPROD Assunto principal: Tétano / Toxoide Tetânico Limite: Humans Idioma: En Revista: Biotechnology Progress Ano de publicação: 2005 Tipo de documento: Article