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Regeneration of Salivary Gland Defects of Diabetic Wistar Rats Post Human Dental Pulp Stem Cells Intraglandular Transplantation on Acinar Cell Vacuolization and Interleukin-10 Serum Level
Narmada, Ida Bagus; Laksono, Valerian; Nugraha, Alexander Patera; Ernawati, Diah Savitri; Winias, Saka; Prahasanti, Chiquita; Dinaryanti, Aristika; Susilowati, Helen; Hendrianto, Eryk; Ihsan, Igo Syaiful; Rantam, Fedik Abdul.
Afiliação
  • Narmada, Ida Bagus; Airlangga Universty. Faculty of Dental Medicine. Department of Orthodontics. Surabaya. ID
  • Laksono, Valerian; s.af
  • Nugraha, Alexander Patera; Airlangga Universty. Faculty of Dental Medicine. Department of Orthodontics. Surabaya. ID
  • Ernawati, Diah Savitri; Airlangga Universty. Faculty of Dental Medicine. Department of Oral Medicine. Surabaya. ID
  • Winias, Saka; Airlangga Universty. Faculty of Dental Medicine. Department of Oral Medicine. Surabaya. ID
  • Prahasanti, Chiquita; Airlangga Universty. Faculty of Dental Medicine. Department of Periodontics. Surabaya. ID
  • Dinaryanti, Aristika; s.af
  • Susilowati, Helen; s.af
  • Hendrianto, Eryk; s.af
  • Ihsan, Igo Syaiful; s.af
  • Rantam, Fedik Abdul; Universitas Airlangga. Faculty of Veterinary Medicine. Department of Microbiology. Surabaya. ID
Pesqui. bras. odontopediatria clín. integr ; 19: e5002, 2019. tab, graf
Article em En | LILACS, BBO | ID: biblio-1056832
Biblioteca responsável: BR1264.1
ABSTRACT
Abstract

Objective:

To investigate the regeneration of rat's salivary gland diabetic defect after intraglandular transplantation of Human Dental Pulp Stem Cells (HDPSCs) on acinar cell vacuolization and Interleukin-10 (IL-10). Material and

Methods:

HDPSCs isolated from the dental pulp of first premolars #34. HDPSCs from the 3rd passage was characterized by immunocytochemistry of CD73, CD90, CD105 and CD45. Twenty-four male Wistar rats, 3-month-old, 250-300 grams induced with Streptozotocin 30 mg/kg body weight to create diabetes mellitus (DM) divided into 4 groups (n=6); positive control group on Day-7; positive control group on Day-14; treatment group Day-7 (DM+5.105HDPSCs); treatment group on Day-14. On Day-7 and Day-14, rats were sacrificed. Histopathological examination performed to analyze acinar cells vacuolization while Enzyme-linked Immunoabsorbent Assay to measure IL-10 serum level. Data obtained were analyzed statistically using multiple comparisons Bonferroni test, Kruskal Wallis, Shapiro-Wilk and Levene's test result

Results:

The highest acinar cell vacuolization found in control group Day 14 (0.239 ± 0.132), meanwhile the lowest acinar cell vacuolization found in treatment group Day 7 (0.019 ± 0.035) with significant difference (p=0.003). The highest IL-10 serum level found in treatment group Day 14 (175.583 ± 120.075) with significant difference (p=0.001)

Conclusion:

Transplantation of HDPSC was able to regenerate submandibular salivary gland defects in diabetic rats by decreasing acinar cell vacuolization and slightly increase IL-10 serum level.
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Texto completo: 1 Coleções: 01-internacional Base de dados: BBO / LILACS Assunto principal: Interleucina-10 / Ratos Wistar / Células-Tronco Totipotentes / Diabetes Mellitus / Células Acinares Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Pesqui. bras. odontopediatria clín. integr Assunto da revista: ODONTOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Indonésia País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: BBO / LILACS Assunto principal: Interleucina-10 / Ratos Wistar / Células-Tronco Totipotentes / Diabetes Mellitus / Células Acinares Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Pesqui. bras. odontopediatria clín. integr Assunto da revista: ODONTOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Indonésia País de publicação: Brasil