lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer

Song, Linlin; Wang, Linlin; Pan, Xiaoli; Yang, Caihong

Song, Linlin; Wang, Linlin; Pan, Xiaoli; Yang, Caihong.

Afiliação

Song, Linlin; General Hospital of Ningxia Medical University. Department of Gynecology. Yinchuan. CN
Wang, Linlin; General Hospital of Ningxia Medical University. Medical Laboratory Center. Yinchuan. CN
Pan, Xiaoli; General Hospital of Ningxia Medical University. Department of Pathology. Yinchuan. CN
Yang, Caihong; General Hospital of Ningxia Medical University. Department of Gynecology. Yinchuan. CN

Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;53(1): e8883, Jan. 2020. tab, graf

Article em En | LILACS | ID: biblio-1055486

Biblioteca responsável: BR1.1

ABSTRACT

ABSTRACT

Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is one kind of cytoplasmic long non-coding RNA (lncRNA), which has been demonstrated to play a critical function in multiple cancers. However, the detailed mechanism of OIP5-AS1 in the regulation of cervical cancer progression is still obscure. Here, we demonstrated that lncRNA OIP5-AS1 was upregulated in cervical cancer and was correlated with poor prognosis by bioinformatics studies. OIP5-AS1 depletion inhibited cell proliferation and promoted cell apoptosis in cervical cancer cells. Furthermore, we clarified that ROCK1 was the downstream effector of OIP5-AS1 and OIP5-AS1 acted as a molecular sponge of miR-143-3p. Finally, we verified that OIP5-AS1 exerted its function in the regulation of cervical cancer progression via interacting with miR-143-3p to regulate ROCK1 expression. Our study revealed novel mechanisms about how lncRNA OIP5-AS1 executed its function in cervical cancer and thus provided potential therapeutic targets for the disease.

Assuntos

Humanos; Feminino; Neoplasias do Colo do Útero/patologia; Apoptose/fisiologia; MicroRNAs/metabolismo; Proliferação de Células/fisiologia; Quinases Associadas a rho/metabolismo; RNA Longo não Codificante/metabolismo; Regulação Neoplásica da Expressão Gênica; Regulação para Cima; Neoplasias do Colo do Útero/metabolismo; Western Blotting; Apoptose/genética; Reação em Cadeia da Polimerase Via Transcriptase Reversa; MicroRNAs/genética; Linhagem Celular Tumoral; Proliferação de Células/genética; Quinases Associadas a rho/genética; RNA Longo não Codificante/genética

Palavras-chave

Cervical cancer; LncRNA; MiR-143-3p; OIP5-AS1; ROCK1

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Neoplasias do Colo do Útero / Apoptose / MicroRNAs / Proliferação de Células / Quinases Associadas a rho / RNA Longo não Codificante Limite: Female / Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

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