A duplex endpoint PCR assay for rapid detection and differentiation of leptospira strains
Rev. Soc. Bras. Med. Trop
; Rev. Soc. Bras. Med. Trop;50(2): 239-242, Mar.-Apr. 2017. tab, graf
Article
em En
| LILACS
| ID: biblio-1041406
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract INTRODUCTION:
This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.METHODS:
Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.RESULTS:
The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water.CONCLUSIONS:
Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
DNA Bacteriano
/
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Leptospira
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Malásia
/
Reino Unido
País de publicação:
Brasil