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Identification of a type I nitroreductase gene in non-virulent Trypanosoma rangeli
Montenegro, Marjorie; Cuervo, Claudia; Cardenas, Constanza; Duarte, Silvia; Díaz, Jenny R; Thomas, M Carmen; Lopez, Manuel C; Puerta, Concepcion J.
Afiliação
  • Montenegro, Marjorie; Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Bogotá. CO
  • Cuervo, Claudia; Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Bogotá. CO
  • Cardenas, Constanza; Pontificia Universidad Católica de Valparaíso. Núcleo de Biotecnología Curauma. Valparaíso. CL
  • Duarte, Silvia; Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Bogotá. CO
  • Díaz, Jenny R; Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Bogotá. CO
  • Thomas, M Carmen; Consejo Superior de Investigaciones Científicas. Instituto de Parasitología y Biomedicina López Neyra. Granada. ES
  • Lopez, Manuel C; Consejo Superior de Investigaciones Científicas. Instituto de Parasitología y Biomedicina López Neyra. Granada. ES
  • Puerta, Concepcion J; Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Bogotá. CO
Mem. Inst. Oswaldo Cruz ; 112(7): 504-509, July 2017. tab, graf
Article em En | LILACS | ID: biblio-1040572
Biblioteca responsável: BR1.1
ABSTRACT
ABSTRACT Trypanosomatid type I nitroreductases (NTRs), i.e., mitochondrial enzymes that metabolise nitroaromatic pro-drugs, are essential for parasite growth, infection, and survival. Here, a type I NTR of non-virulent protozoan Trypanosoma rangeli is described and compared to those of other trypanosomatids. The NTR gene was isolated from KP1(+) and KP1(-) strains, and its corresponding transcript and 5' untranslated region (5'UTR) were determined. Bioinformatics analyses and nitro-drug activation assays were also performed. The results indicated that the type I NTR gene is present in both KP1(-) and KP1(+) strains, with 98% identity. However, the predicted subcellular localisation of the protein differed among the strains (predicted as mitochondrial in the KP1(+) strain). Comparisons of the domains and 3D structures of the NTRs with those of orthologs demonstrated that the nitroreductase domain of T. rangeli NTR is conserved across all the strains, including the residues involved in the interaction with the FMN cofactor and in the tertiary structure characteristics of this oxidoreductase protein family. mRNA processing and expression were also observed. In addition, T. rangeli was shown to be sensitive to benznidazole and nifurtimox in a concentration-dependent manner. In summary, T. rangeli appears to have a newly discovered functional type I NTR.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Nitrorredutases / Trypanosoma rangeli Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Chile / Colômbia / Espanha País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Nitrorredutases / Trypanosoma rangeli Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Chile / Colômbia / Espanha País de publicação: Brasil