Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma

Yang, Ju-Hong; Lin, Lie-Kun; Zhang, Song

Yang, Ju-Hong; Lin, Lie-Kun; Zhang, Song.

Afiliação

Yang, Ju-Hong; University of Chinese Academy Sciences-Shenzhen Hospital. Central Laboratory. Shenzhen. CN
Lin, Lie-Kun; University of Chinese Academy Sciences-Shenzhen Hospital. Central Laboratory. Shenzhen. CN
Zhang, Song; University of Chinese Academy Sciences-Shenzhen Hospital. Department of Otolaryngology. Shenzhen. CN

Biol. Res ; 52: 31, 2019. graf

Article em En | LILACS | ID: biblio-1019497

Biblioteca responsável: CL1.1

ABSTRACT

ABSTRACT

BACKGROUND:

The purpose of the present study was to investigate the role of the methylation status of the DACT1 gene on the invasion and metastasis of nasopharyngeal carcinoma cells.

METHODS:

The levels of methylation and expression of the DACT1 gene in nasopharyngeal carcinoma tissues and CNE2 cells were determined by methylation-specific PCR and RT-PCR, respectively. CNE2 cells were treated with 5-aza-2-deoxycytidine, and the variation in the methylation status of the DACT1 gene was detected, as well as the influence of methylation on invasiveness of nasopharyngeal carcinoma cells.

RESULTS:

The DACT1 gene was hyper-methylated in 44 of 62 cases of nasopharyngeal carcinoma. The DACT1 gene was hyper-methylated in 32 of 38 cases of nasopharyngeal carcinoma with lymph node metastasis, and the DACT1 gene was hyper-methylated in 7 of 24 cases of nasopharyngeal carcinoma without lymph node metastasis. The DACT1 mRNA level was weakly expressed or not expressed in all nasopharyngeal carcinoma tissues with hyper-methylated DACT1 genes; however, the DACT1 mRNA level was highly expressed in nasopharyngeal carcinoma tissues with low expression of the methylated DACT1 gene. The DACT1 gene was hyper-methylated and not expressed in CNE2 cells that did not have 5-aza-2-deoxycytidine treatment. After 5-aza-2-deoxycytidine treatment, the DACT1 gene was demethylated and the expression of DACT1 was restored. Moreover, the invasion ability was inhibited in CNE2 cells treated with 5-aza-2-deoxycytidine.

CONCLUSION:

The expression of DACT1 was related to the methylation status. High expression of DACT1 may inhibit the invasion and metastasis of nasopharyngeal carcinoma cells.

Assuntos

Humanos; Masculino; Feminino; Proteínas Nucleares/genética; Neoplasias Nasofaríngeas/patologia; Metilação de DNA/genética; Proteínas Adaptadoras de Transdução de Sinal/genética; Carcinoma Nasofaríngeo/secundário; Proteínas Nucleares/metabolismo; Neoplasias Nasofaríngeas/genética; Regiões Promotoras Genéticas; Metilação de DNA/fisiologia; Proteínas Adaptadoras de Transdução de Sinal/metabolismo; Carcinoma Nasofaríngeo/genética; Invasividade Neoplásica; Proteínas de Neoplasias/metabolismo

Palavras-chave

5-Aza-2-deoxycytidine; CNE2 cell line; DACT1 gene; Invasion; Metastasis; Nasopharyngeal carcinoma

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Proteínas Nucleares / Neoplasias Nasofaríngeas / Metilação de DNA / Proteínas Adaptadoras de Transdução de Sinal / Carcinoma Nasofaríngeo Limite: Female / Humans / Male Idioma: En Revista: Biol. Res Assunto da revista: BIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China País de publicação: Chile

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