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Generation of dimeric single-chain antibodies neutralizing the cytolytic activity of vaginolysin
Dapkunas, Zilvinas; Baranauskas, Aurimas; Mickiene, Gitana; Pleckaityte, Milda; Zvirblis, Gintautas.
Afiliação
  • Dapkunas, Zilvinas; Vilnius University. Institute of Biotechnology. Vilnius. LT
  • Baranauskas, Aurimas; Profarma UAB. Vilnius. LT
  • Mickiene, Gitana; Vilnius University. Institute of Biotechnology. Vilnius. LT
  • Pleckaityte, Milda; Vilnius University. Institute of Biotechnology. Vilnius. LT
  • Zvirblis, Gintautas; Vilnius University. Institute of Biotechnology. Vilnius. LT
Electron. j. biotechnol ; Electron. j. biotechnol;28: 52-57, July. 2017. ilus, graf, tab
Article em En | LILACS | ID: biblio-1015847
Biblioteca responsável: CL1.1
ABSTRACT

Background:

Gardnerella vaginalis is a bacterial vaginosis (BV)-associated vaginal bacterium that produces the toxin vaginolysin (VLY). VLY is a pore-forming toxin that is suggested to be the main virulence factor of G. vaginalis. The high recurrence rate of BV and the emergence of antibiotic-resistant bacterial species demonstrate the need for the development of recombinant antibodies as novel therapeutic agents for disease treatment. Single-chain variable fragments (scFvs) generated against VLY exhibited reduced efficacy to neutralize VLY activity compared to the respective full-length antibodies. To improve the properties of scFvs, monospecific dimeric scFvs were generated by the genetic fusion of two anti-VLY scFv molecules connected by an alpha-helix-forming peptide linker.

Results:

N-terminal hexahistidine-tagged dimeric scFvs were constructed and produced in Escherichia coli and purified using metal chelate affinity chromatography. Inhibition of VLY-mediated human erythrocyte lysis by dimeric and monomeric scFvs was detected by in vitro hemolytic assay. The circulating half-life of purified scFvs in the blood plasma of mice was determined by ELISA. Dimeric anti-VLY scFvs showed higher neutralizing potency and extended circulating half-life than parental monomeric scFv.

Conclusions:

The protein obtained by the genetic fusion of two anti-VLY scFvs into a dimeric molecule exhibited improved properties in comparison with monomeric scFv. This new recombinant antibody might implement new possibilities for the prophylaxis and treatment of the diseases caused by the bacteria G. vaginalis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Proteínas de Bactérias / Toxinas Bacterianas / Anticorpos Neutralizantes / Anticorpos de Cadeia Única Limite: Animals Idioma: En Revista: Electron. j. biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2017 Tipo de documento: Article / Project document País de afiliação: Lituânia País de publicação: Chile

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Proteínas de Bactérias / Toxinas Bacterianas / Anticorpos Neutralizantes / Anticorpos de Cadeia Única Limite: Animals Idioma: En Revista: Electron. j. biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2017 Tipo de documento: Article / Project document País de afiliação: Lituânia País de publicação: Chile