Expression analysis of long non-coding RNAs in a renal ischemia-reperfusion injury model
Acta cir. bras
; Acta cir. bras;34(4): e201900403, 2019. tab, graf
Article
em En
| LILACS
| ID: biblio-1001087
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the long non-coding RNAs (lncRNAs) profile on renal ischemia reperfusion in a mouse model.Methods:
Microarray analysis was used to study the expression of misregulated lncRNA in a mouse model of renal ischemia reperfusion(I/R) with long ischemia time. Quantitative real-time PCR (qPCR) was used to verify the expression of selected lncRNAs and mRNAs.The potential functions of the lncRNA was analyzed by bioinformatics tools and databases.Results:
Kidney function was impaired in I/R group compared to the normal group. Analysis showed that a total of 2267 lncRNAs and 2341 messenger RNAs (mRNAs) were significantly expressed in I/R group (≥2.0-fold, p < 0.05).The qPCR result showed that lncRNAs and mRNAs expression were consistent with the microarray analysis. The co-expression network profile analysis based on five validated lncRNAs and 203 interacted mRNAs showed it existed a total of 208 nodes and 333 connections. The GO and KEEG pathway analysis results showed that multiple lncRNAs are involved the mechanism of I/R.Conclusion:
Multiple lncRNAs are involved in the mechanism of I/R.These analysis results will help us to further understand the mechanism of I/R and promote the new methods targeted at lncRNA to improve I/R injury.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
RNA Mensageiro
/
Traumatismo por Reperfusão
/
RNA Longo não Codificante
/
Rim
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
Brasil