Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci / 法医学杂志
Journal of Forensic Medicine
; (6): 373-380, 2015.
Article
en Zh
| WPRIM
| ID: wpr-984016
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice.@*METHODS@#Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed.@*RESULTS@#A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin.@*CONCLUSION@#The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Cartilla de ADN
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Repeticiones de Microsatélite
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Bases de Datos de Ácidos Nucleicos
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Cromosomas Humanos Y
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Amelogenina
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Genética Forense
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Técnicas de Genotipaje
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Reacción en Cadena de la Polimerasa Multiplex
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Genotipo
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Indicadores y Reactivos
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2015
Tipo del documento:
Article