Extract human DNA from maggot crop contents by phenol-chloroform method coupled with paramagnetic particle method / 法医学杂志
Journal of Forensic Medicine
; (6): 421-424, 2009.
Article
en Zh
| WPRIM
| ID: wpr-983516
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.@*METHODS@#Human DNA was extracted from the maggot crop contents using phenol-chloroform method and purified by paramagnetic particle method. DNA was quantified by PCR with Quantifiler Human DNA Quantification Kit using 7500 real-time fluorescence quantitative PCR instrument. PCR products were genotyped by AmpFlSTR Identifiler PCR Amplification Kit using 3130XL-Avant genetic analyzer.@*RESULTS@#The template DNA yield by the method described were increased at least 2 times than the phenol-chloroform extraction method alone. All of the full 16 STR profiles could be obtained with the samples extracted by this method when the DNA yield reached (0.218 +/- 0.041) ng/microL.@*CONCLUSION@#Phenol-chloroform method coupled with paramagnetic particle method can effectively increase the sensitivity of STR analysis of human DNA recovered from maggot crop contents and is a valuable tool for forensic entomology.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Cadáver
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ADN
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Cloroformo
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Reacción en Cadena de la Polimerasa
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Dermatoglifia del ADN
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Sensibilidad y Especificidad
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Fenol
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Secuencias Repetidas en Tándem
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Ciencias Forenses
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Dípteros
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2009
Tipo del documento:
Article