Application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies / 法医学杂志
Journal of Forensic Medicine
; (6): 411-413, 2007.
Article
en Zh
| WPRIM
| ID: wpr-983328
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies.@*METHODS@#The cerebrum, cerebellum, brainstem, and hippocampus of four patients died of rabies identified by clinical diagnosis were collected and kept in freezer at -70 degrees C or in formaldehyde solution separately. The rat brain tissue infected by CVS strain of rabies virus was used as a positive control and the brain tissue of a patient died of acute pancreatitis was used as a negative control.@*RESULTS@#Rabies virus was detected in the tissues kept in freezer at -70 degrees C and the positive control but was not detected in the tissues kept in formaldehyde solution and the negative control.@*CONCLUSION@#Immunofluorescence and Sandwich ELISA with double-antibodies could be used in detection of human rabies. The samples should be kept in deep frozen temperature condition instead of in formaldehyde solution.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Rabia
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Virus de la Rabia
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Juego de Reactivos para Diagnóstico
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Conservación de Tejido
/
Encéfalo
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Ensayo de Inmunoadsorción Enzimática
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Técnica del Anticuerpo Fluorescente
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Sensibilidad y Especificidad
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Hipocampo
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Anticuerpos Antivirales
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2007
Tipo del documento:
Article