Cellular mechanism of heart injury in the early stage of crush injury in rats / 法医学杂志
Journal of Forensic Medicine
; (6): 90-92, 2006.
Article
en Zh
| WPRIM
| ID: wpr-983146
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To study cellular mechanism of cardiomyocytes injury in the early stage of crush injury by observing some effects of crush injury rat sera on cultured neonatal rat cardiomyocytes.@*METHODS@#One to three days old neonatal rat cardiomyocytes were cultured in vitro and some effects of crush injury rat sera on beating rate, cell surface area, total protein content, 3H-Leu incorporation, intracellular calcium concentration ([Ca2+]i) and Fos protein expression were observed in cultured rat cardiomyocytes.@*RESULTS@#Compared with normal rat serum group, crush injury rat sera decreased beating rate(beats/min) of cardiomyocytes from 88.3 to 26.4, cell surface area, total protein content, 3H-Leu incorporation, [Ca2+]i (nmol/L) and PI of Fos protein expression were increased.@*CONCLUSION@#Crush injury rat sera suppress cell beating, increase intracellular calcium, induce Fos protein synthesis and cause cell hypertrophy, which may cause cardiac injury in the early stage of rush injury.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Células Cultivadas
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Calcio
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Proteínas Proto-Oncogénicas c-fos
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Ratas Sprague-Dawley
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Miocitos Cardíacos
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Tamaño de la Célula
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Modelos Animales de Enfermedad
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Extremidades
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Lesiones Cardíacas
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Frecuencia Cardíaca
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2006
Tipo del documento:
Article