Preliminary study of tumor cells and bio-engineering cells carried by returned satellite / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
; (12): 644-646, 2004.
Article
en Zh
| WPRIM
| ID: wpr-979513
Biblioteca responsable:
WPRO
ABSTRACT
@#ObjectiveTo study the effects on growth characteristics of cells mutated by outer space. Methods5 tumor cell lines and 1 bio-pharmaceutical cell line were carried in the recoverable satellite No.18 and returned after 18 days flying in outer space.The cell living systems were utilized in passive carrying. Some survival cells were monocoloned, and the experimental methods such as cell morphological observation,MTT assay and FACS were used for analysis of cell growth characteristics. ResultsThe survival cell rates of tumor cell lines and bio-pharmaceutical cells were markedly different. Compared with the control group, mutated cells appeared to have multiple cell morphological changes and the cell number of G1 phase was increased markedly (P<0.05). Growth rate in mutated cells were varied without specific discipline. ConclusionEffects of outer space on growth characteristics were complicated and multiplex in mutated cells,which were not all heredity in further passage. The differences of the monoclonal cell lines provide the potential to obtain optimizing cell lines by screen.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Rehabilitation Theory and Practice
Año:
2004
Tipo del documento:
Article