RNA Interference Targeting c-Met Inhibits Proliferation of Lens Epithelial Cells / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
; (12): 442-444, 2008.
Article
en Zh
| WPRIM
| ID: wpr-965768
Biblioteca responsable:
WPRO
ABSTRACT
@#Objective To observe the effect of small-interfering RNA(siRNA) targeting c-Met,the receptor of hepatocyte growth factor(HGF),on the proliferation of human lens epithelial cells(LECs).Methods siRNA was transferred into LECs cultured in vitro by HiperFect Transfection Reagent.Real-Time PCR was applied to observe the expression of c-Met mRNA in LECs after gene transfer,and MTT assay was used to detect the proliferation of LECs induced by HGF.Results The expression of c-Met mRNA in LECs was significantly decreased in the experimental group,compared to that in the controls(P<0.01).Proliferation of LECs induced by HGF was inhibited,compared with the single HGF stimulated group(P<0.01).Conclusion The RNA interference targeting c-Met can effectively inhibit the expression of c-Met mRNA,and the proliferation of LECs induced by HGF.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Rehabilitation Theory and Practice
Año:
2008
Tipo del documento:
Article